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Ecotoxicological information

Toxicity to microorganisms

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Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1987
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
The main structure of the read-across substance is the same of the CJ313, only the salt form is different. However, this difference does not influence the toxicity of the CJ313. CJ313 contains Li/Na salt form and the read-across substances contains only Na salt. This will not lead to difference on the toxicity results.
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
Principles of method if other than guideline:
None
GLP compliance:
no
Specific details on test material used for the study:
None
Analytical monitoring:
yes
Details on sampling:
None
Vehicle:
not specified
Details on test solutions:
None
Test organisms (species):
activated sludge
Details on inoculum:
None
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Remarks on exposure duration:
None
Post exposure observation period:
None
Hardness:
None
Test temperature:
None
pH:
None
Dissolved oxygen:
None
Salinity:
None
Conductivity:
None
Nominal and measured concentrations:
100.0, 32.0 and 10.0 mg/l
Details on test conditions:
None
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
> 400 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Details on results:
None
Results with reference substance (positive control):
The IC50 of reference substance (3,5-Dichlorophenol) = 13 mg/l.
Reported statistics and error estimates:
None
Validity criteria fulfilled:
not specified
Conclusions:
The IC50 for FAT 40032/D was found to be greater than 400 mg/L.
Executive summary:

A key toxicity study of FAT 40032/D to activated sludge was determined in a 3-hours respiration inhibition test following methodology similar to OECD Guideline 209. At 10, 32 and 100 mg/L concentrations, respiratory inhibitions observed were 0, 6.3 and 15.8 % respectively. Based on the findings of the study, it was concluded that the IC50 (3 h) of the test substance FAT 40032/D is greater than 400 mg/L.

Description of key information

The IC50 for FAT 40032/D was found to be greater than 400 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
400 mg/L

Additional information

The IC50 for Reactive Brown 11 was reported to be >300 mg/L in the four available study reports.

In the key toxicity study of FAT 40032/D to activated sludge, conducted according to methodology similar to OECD Guideline 209. In this 3-hours respiration inhibition test, the test substance concentrations tested were 100.0, 32.0 and 10 mg/l. Based on the findings of the study, the IC50 (3 h) of the test substance FAT 40032/D was found to be >400 mg/L.

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