Dibenzo[d,f][1,3,2]dioxaphosphepin, 6,6'-[[3,3',5,5'-tetrakis(1,1-dimethylethyl)[1,1'-biphenyl]-2,2'-diyl]bis(oxy)]bis-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study with analytical test item concentration verification

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

L4-Ligand

IUPAC Name:

L4-Ligand

Constituent 2

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): L4-Ligand

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

2ml of test solution was taken from each test vessel at the beginning and at the end of the test.

Test solutions

Vehicle:

no

Details on test solutions:

0.5032 g of the test substance was added in 5L test water at the loading rate of 100 mg/L. The mixtures was stirred (via magnetic stirrer) in the dark for 72h at 500rpm followed by filtration using a 0.45 μm nitrocellulose membrane.

Test organisms

Test organisms (species):

other: Gobiocypris rarus

Details on test organisms:

TEST ORGANISM
- Common name: Rare minnow
- Source: bred in Bioassay and Safety Assessment Lab, Shanghai Academy of Public Measurement, China
- Age at study initiation (mean and range, SD): about 4 month
- Length at study initiation (length definition, mean and SD): 2.3 +/- 0.1 cm
- Weight at study end (mean and range, SD): 0.22 +/- 0.03 g
- Fish were fed daily until 24 hours before the test was started

ACCLIMATION
- all fish were acclimatized for more than 14 days
- Conditions equivalent to housing
- Health during acclimation (any mortality observed): no mortality within 7 days before test initiation, no diseases were observed during the acclimation period
- Not medically treated before test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

Total hardness: 145 mg CaCO3/L
Dissolved oxygen concentration: >90% of ASV

Test temperature:

23.7 - 24.2 °C

pH:

8.4 - 8.6

Dissolved oxygen:

5.5 - 8.2 mg/L

Nominal and measured concentrations:

Nominal: 100 mg/L loading
Both the LOD2 and LOQ2 were well below the reported water solubility <0.1 mg/L.

Details on test conditions:

TEST SYSTEM
- Test vessel: 5L glass tanks containing 4L test solution
- Loading: about 0.5g fish/litre
- Feeding: none

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water, dechlorinated by activated carbon and sterilized by UV lamp, aerated continuously over 24h.

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light : 12 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :0, 24, 48, 72 and 96 hours after test initiation mortalities, abnormalities, pH , dissolved oxygen and temperature; at test end, body length and body weight of the test fish of the control group were measured.

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Measured concentration of 100 mg/L loading was below LOD2 (39.6 ng/mL). There was no acute toxicity of the saturated solution in the limit test.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

There were no mortalities and abnormalities observed in the fish of the control group and the treatment group during the test.