Registration Dossier
Registration Dossier
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EC number: 269-905-8 | CAS number: 68390-58-9
Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Media:
- soil/sewage sludge
- Test temperature:
- 25 °C (column temperature)
- Details on study design: HPLC method:
- EQUIPMENT
- Apparatus:
- Type: Agilent 1200 Series Liquid Chromatograph
- Type, material and dimension of analytical (guard) column: HyperClone 5µ CN (CPS) (25 cm x 4.6 mm internal diameter)
- Detection system: UV set at 210 nm
MOBILE PHASES
- Type: Acetonitrile:water (55:45 v/v)
- Experiments with additives carried out on separate columns: no additives used
- pH: no data
- Solutes for dissolving test and reference substances: HPLC mobile phase
DETERMINATION OF DEAD TIME
- Method: by inert substances which are not retained by the column (formamide)
REFERENCE SUBSTANCES
- Identity: Acetanilide, Nitrobenzene, Naphthalene, 2-Methylnaphthalene, Anthracene, DDT
- Known logKoc values: 1.3, 2.4, 2.8, 3.9, 4.4, 5.6
DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: ca. 2 µg
- Quantity of reference substances:
Formamide (internal deadtime standard): 2 µg, Acetanilide: 0.2 µg, Nitrobenzene: 0.2 µg, Naphthalene: 0.05 µg, 2-Methylnaphthalene: 0.05 µg, Anthracene: 0.2 µg, DDT: 0.2 µg
- Intervals of calibration: before and after injections of the test substance
REPETITIONS
- Number of determinations: two
EVALUATION
- Determination of the log Koc value: A calibration plot of log10k versus log10Koc was constructed for the reference standards. The logarithms of the capacity factors for the test substance were calculated and the log10Koc values were determined by interpolation. - Key result
- Type:
- log Koc
- Value:
- >= 4.3 - <= 5.2
- Temp.:
- 25 °C
- Details on results (HPLC method):
- The main components of the test material were found to have LogKoc values between 4.3 and 5.2.
- Retention times of reference substances used for calibration:
acetanilide (3.4 min), nitrobenzene (4.2), naphthalene (5.0), 2-methylnaphthalene (5.3), anthracene (6.0), DDT (9.1)
- Details of fitted regression line: log10Koc = 4.48 log10k + 3.86 - Validity criteria fulfilled:
- not applicable
Reference
Description of key information
Log Koc at 25 °C: 4.3-5.2 (HPLC method; OECD 121, EU C.19)
Key value for chemical safety assessment
Additional information
The main components of the test material were found to have LogKoc values between 4.3 and 5.2.
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