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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
Directive 84/449/EEC, C2
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
1,2-epoxybutane
EC Number:
203-438-2
EC Name:
1,2-epoxybutane
Cas Number:
106-88-7
Molecular formula:
C4H8O
IUPAC Name:
2-ethyloxirane
Details on test material:
CHEMICAL NAME: 1,2-epoxybutane
PURITY: 99.9 %

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
- source: Daphnia magna Straus was originally supplied from "Institut National de Recherche Chimique appliquee, France", and has been breeded from 1978 in the Laboratories of BASF
- age: 2 - 24 hours old

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
total hardness = 2.70
pH:
pH range over all concentrations and 0/48h: 7.71 - 7.95
Dissolved oxygen:
dissolved oxygen range over all concentrations and 0/48h: 8.15 - 8.71 mg/L
Details on test conditions:
TEST CONDITIONS
- dilution water chemistry: according to guideline
- test water specifications: pH = 7.7 - 8.3
- conductivity = 550 - 650 uSiemens/cm,
- exposure vessels: reagent tubes with flat bottom
- 10 ml test volume
- light conditions: 16:8 hours, diffuse light (5 microeinstein/m*m*s (400-750 nm))
- number of replicates: four
- individuals / replicate: five
- dilution series with dilution water gave the following nominal test concentrations: 7.81, 15.6, 31.2, 62.5, 125, 250 and 500 mg/l.

STATISTICS
- moving average was used to calculate EC 50 (48h)
- method of Spearman Kaerber was used to calculate EC 50 (24h)

MEASUREMENTS
- pH values and dissolved oxygen: After 0 and 48 hours
- mobile daphnids: after 0, 3, 6, 24 and 48 hours

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
31 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
70 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 48.95 - 90.67
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
125 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

CONCENTRATION RESPONSE RELATIONSHIP
Mobile daphnids after 24 hours:
Concentration	0h	3h	6h	24h	48h
  0    mg/L      20	20	20	20	20	
  7.81 mg/L	20	20	20	20	20 
 15.6  mg/L	20	20	20	20	19 
 31.2  mg/L	20	20	20	20	19 
 62.5  mg/L	20	20	20	20	14 
125    mg/L	20	20	20	17	 0 
250    mg/L	20	20	20	 0	 0 
500    mg/L	20	20	19	 0	 0

EC-VALUES [mg/L]
24h-EC0   =  62.5
24h-EC50  = 159.66; 95% confidence level (142.87 - 178.42)
24h-EC100 = 250

Applicant's summary and conclusion