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EC number: 304-802-4 | CAS number: 94279-56-8
Silicic acid (H4SiO4), zirconium (4+) salt (1:1), reaction products with sodium hydroxide was tested in theSalmonella typhimuriumreverse mutation assay with four histidine-requiring strains ofSalmonella typhimurium(TA1535, TA1537, TA98 and TA100) and in theEscherichia colireverse mutation assay with a tryptophan-requiring strain ofEscherichia coli(WP2uvrA). Initially a dose range finding test was performed with the tester strains TA100 and WP2uvrA. After that the test was performed in two independent experiments in the presence and absence of S9-mix (rat liver S9-mix induced by Aroclor 1254).
BatchCP2684of the test item was a brown powder. The test item was suspended in dimethyl sulfoxide. No workable suspension above 10 mg/ml could be obtained.
In the dose range finding test, the test item was tested up to concentrations of 1000 µg/plate in the absence and presence of S9-mix in the strains TA100 and WP2uvrA. The test item did not precipitate on the plates at this dose level. The bacterial background lawn was not reduced at any of the concentrations tested and no biologically relevant decrease in the number of revertants was observed.
Based on the results of the dose range finding test, the test item was tested in the first mutation assay at a concentration range of 52 to 2500 µg/plate in the absence and presence of 5% (v/v) S9-mix in the tester strains TA1535, TA1537, TA98, TA100 and WP2uvrA. The test item precipitated on the plates at the top dose of 2500 μg/plate. Cytotoxicity, as evidenced by a decrease in the number of revertants, was observed in tester strain TA98 in the absence of S9-mix at the highest tested concentration.
In a follow-up experiment of the assay with additional parameters, the test item was tested at a concentration range of 200 to 2500 µg/plate in the absence and presence of 10% (v/v) S9-mix in the tester strains TA1535, TA1537, TA98, TA100 and WP2uvrA. The test item precipitated on the plates at the top dose of 2500 μg/plate. Toxicity was observed in tester strain TA98 in the absence of S9-mix at the highest tested concentration.
Silicic acid (H4SiO4), zirconium (4+) salt (1:1), reaction products with sodium hydroxide did not induce a significant dose-related increase in the number of revertant (His+) colonies in each of the four tester strains (TA1535, TA1537, TA98 and TA100) and in the number of revertant (Trp+) colonies in the tester strain WP2uvrA both in the absence and presence of S9-metabolic activation. These results were confirmed in a follow-up experiment.
In this study, acceptable responses were obtained for the negative and strain-specific positive control items indicating that the test conditions were adequate and that the metabolic activation system functioned properly.
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