Tetrakis(hydroxymethyl)phosphonium chloride, oligomeric reaction products with urea

Additional toxicological data

Administrative data

Endpoint:

additional toxicological information

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1980

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Scientific paper. Refers to other scientific papers for method descriptions. May be in accordance with the later OECD or EU guidelines, but it is uncertain.

Cross-reference

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1980

Materials and methods

Type of study / information:

Extracts tested in three assays:
-V79 Cell Mutagenesis Assay
-Agar Suspension Transformation Assay
-3T3 Cell Transformation Assay
Only data relevant for Proban CC is cited.

Test guideline

Qualifier:

no guideline followed

Deviations:

not applicable

Principles of method if other than guideline:

V79 cell mutation assay was performed accorcing to Kuroki et al. (1977) and Langenbach et al. (1978).
BHK 21/Cl 13 agar suspension transformation assay was performed according to MacPherson et al. (1966) and Styles (1977).
3T3 cell transformation assay was performed according to Kakunaga (1973).

GLP compliance:

no

Test material

Test material information

Constituent 1

Details on test material:

Extracts from cotton textile treated with THPC-urea precondensate (condensation product between tetrakis(hydroxymethyl)phosphonium chloride and urea, not further characterised) and subsequently cured with ammonia gas.

Results and discussion

Any other information on results incl. tables

Effect on mutagenesis in V79 cells of DMSO extracts of cotton fabrics treated with THPC-urea precondensate.

		Survival (% of control)			Ouabain-resistant mutants per 10" survivors
		- S9	- S9		- S9	- S9
Treatment	Concentration	Exp. I	Exp. 2	+ S9	Exp. I	Exp.2	+ S9
DMSO	0.5%	100	100	100	0	0	0
Extract unconcentrated	0.5%	98	108	83	22	0	44
Extract 10-fold concentrated	0.5%	35	21	88	113	71	24

Effect on colony-forming ability of BHK cells in agar of DMSO extracts of cotton fabrics treated with THPC-urea precondenate.

		Survival (% of control) In experiment^a		Average number of colonies per plate in experiment		Estimated number of colonies per 10^6 survivors in experiment
Treatment	Concentration	1	2	1	2	1	2
DMSO	0.5%	102	100	27	35	31	35
Extract unconcentrated	0.5%	85	107	37	106	43	102
Extract 10 -fold concentrated	0.5%	0	0	4	23	> 660	>2230

a) Experiment 1 was performed without S9 and experiment 2 with S9

3T3 Cell Transformation Assay of Extracts of Flame Retardant-treated Cotton Fabrics^a

				Transformed foci per 8 plates				Transformed foci per 10^5 surviving cells^b
				3T3 (ATTC)		3T3 (A31-714)		3T3 (ATTC)		3T3 (A31-714)
Treatment	Concentration	3T3 (ATTC)	3T3 (A31-714)	Exp. 1	Exp. 2	Exp. 3	Exp. 4	Exp. 1	Exp. 2	Exp. 3	Exp. 4
DMSO	0.5%	31	13	0	4	1	0	0	16	10	0
Extract unconcentrated	0.5%	31	13	13	2	7	NT	21	8	67	NT
Extract 10-fold concentrated	0.5%	2.5	0.5	12	7	4	NT	240	350	1000	NT

a) in experiment 1 - 2.5x10⁴ cells were treated per plate; in experiments 2 and 3 - 1x10⁴ cells; in experiment 4 - 5x10⁴ cells. In experiment 4 only samples that showed no evidence of precipitation were used.

b) survival measured by cloning efficiency; actual values may be lower if survival increases with number of cells planted.

NT=not tested

Applicant's summary and conclusion

Conclusions:

The results show that components in the concentrated extracts are quite toxic to BHK and 3T3 cells, but less toxic to V79 cells. The unconcentrated extracts, which are almost not cytotoxic, are mutagenic and transforming. The data do not relate the mutagenic and transforming effects to actual components; thus the response cannot be quantified. It has been not determined whether the toxic components were formed during the concentration or storage of the samples. All tested samples are extracts from dyed fabrics treated with THPC-urea precondensate and subsequently cured with ammonia gas. Therefore, the samples may also contain dyes extracted from the fabrics. No precise conclusion can be drawn from present study about the transforming or mutagenic potential of THPC-urea precondensates.