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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Key value for chemical safety assessment

Additional information

One Ames test (Marzin 1994) was available on the mutagenic properties of different hydrocarbon substances. No mutagenic effect was obseved without and with metabolic activation.

A mouse Mammalian Erythrocyte Micronucleus performed with kerosene also showed no mutagenic properties (American Petroleum Institute, 1984).

Structurally analogous test materials, hydrodesulfurized kerosene and jet fuel A were also non-mutagenic when tested in in vivo studies: mouse bone marrow micronucleus assay, mammalian bone marrow chromosome aberration test and a dominant lethal assay. 

One bone marrow micronucleus assay using CD-1 mice.  The test materials were tested at 24, 48, and 72 hour intervals following exposure and did not induce a statistically significant decrease in the mean percent of polychromatic erythrocytes or an increase in the mean number of micronucleated polychromatic erythrocytes. Both the positive (cyclophosphamide) and the negative (carrier) controls behaved in an appropriate manner.  These data indicate that kerosenes are not cytotoxic and are not clastogenic in CD-1 mouse bone marrow cells at doses up to and including 5.0 g/kg of body weight.


Short description of key information:
Ames tests on Renewable hydrocarbons, C17-C18, branched alkanes showed no mutagenic effect with and without metabolic activation. The chromosome aberration study in CHO cells on "hydrocarbons, C12-C16, n-alkanes, isoalkanes, cyclics,<2% aromatics" also showed no signs of mutagenicity. A mouse Mammalian Erythrocyte Micronucleus performed with kerosene also showed no mutagenic properties.

Endpoint Conclusion: No adverse effect observed (negative)

Justification for classification or non-classification

All in vitro and in vivo tests on test substances and analogues were negative, the substances are considered not to be mutagenic and should not be classified for mutagenicity according to the criteria of Annex VI of Directive 67/548/EEC and CLP Regulation 1272/2008.