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EC number: 249-694-9 | CAS number: 29556-33-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Data is from study report
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Principles of method if other than guideline:
- Dermal toxicity of 2-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl] benzoxazole-5-sulphonamide (CAS No. 68427-35-0) after single dose application by dermal route in rats and an observation period of 14 days.
- GLP compliance:
- yes
- Test type:
- other: Acute dermal Toxicity
- Limit test:
- yes
Test material
- Reference substance name:
- 2-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl]benzoxazole-5-sulphonamide
- EC Number:
- 270-393-3
- EC Name:
- 2-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl]benzoxazole-5-sulphonamide
- Cas Number:
- 68427-35-0
- Molecular formula:
- C20H19N3O5S
- IUPAC Name:
- 2-[7-(diethylamino)-2-oxo-2H-chromen-3-yl]-1,3-benzoxazole-5-sulfonamide
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Name of test material : 2-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl] benzoxazole-5-sulphonamide
- Molecular formula : C20H19N3O5S
- Molecular weight :413.452 g/mole
- Substance type: Organic
- Physical state:Powder
- Source and lot/batch No.of test material:FG/15-16/1865
Constituent 1
- Specific details on test material used for the study:
- - Name of test material : 2-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl] benzoxazole-5-sulphonamide
- Molecular formula : C20H19N3O5S
- Molecular weight :413.452 g/mole
- Substance type: Organic
- Physical state:Powder
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: In-House Bred at sa-FORD, Animal Facility (CPCSEA Registration No. 1256/bc/09/CPCSEA)
- Age at study initiation: No data available
- Weight at study initiation: Male:Minimum: 240 g and Maximum: 280 g , Female:Minimum: 222 g and Maximum: 239 g
- Fasting period before study:No data available
- Housing: Animals were housed three per polycarbonate cage of size 37 [cm] x 21 [cm], height 20 [cm] and identified by toe pad micro tattooing and cage cards. Individual cage cards were labelled with study no., study type, test system, group, dose, sex, animal number experimental start date, dosing date and completion date.
- Diet (e.g. ad libitum): conventional laboratory rodent diet (Nutrivet Life Sciences, Pune) ad libitum. Batch No: 040316., ad libitum
- Water (e.g. ad libitum): Aqua guard filtered tap water, ad libitum
- Acclimation period:7 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C):Minimum: 19.80 °C - Maximum: 22.80 °C
- Humidity (%):Minimum: 47.10% - Maximum: 68.60%
- Air changes (per hr):12 hour light and 12 hour dark
- Photoperiod (hrs dark / hrs light):More than 12 changes per hour
IN-LIFE DATES: From: April 19, 2016
To:May 03, 2016
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- other: distilled water
- Details on dermal exposure:
- TEST SITE
- Area of exposure: the fur of dorsal area of the trunk of rats was clipped by using clipper.
- % coverage: greater than 10% body surface area
- Type of wrap if used: a porous gauze dressing and non-irritating tape
Details on exposure:
VEHICLE
- Concentration in vehicle: 2000 mg/kgbw
- Amount of vehicle (if gavage): 0.2 ml
- Justification for choice of vehicle:No data available
- Lot/batch no. (if required):No data available
- Purity:No data available
MAXIMUM DOSE VOLUME APPLIED:2000 mg/kgbw - Duration of exposure:
- 24 hour
- Doses:
- 2000 mg/kg body weight
- No. of animals per sex per dose:
- 5 male, 5 female
- Control animals:
- not specified
- Details on study design:
- Details on study design
- Duration of observation period following administration: 14 day observation period
- Frequency of observations and weighing: at 1, 2, 3 and 4 hours post dosing on day 0 (day of dosing) and once a day during the 14 day observation period.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other: Yes - Statistics:
- No statistical analysis was performed since the study was terminated with limit test
Results and discussion
- Preliminary study:
- no data available
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: no mortality was observed
- Mortality:
- No mortality was observed at limit dose of 2000 mg/kg body weight of test item during the 14 day observation period
- Clinical signs:
- other: No systemic signs of toxicity were observed at limit dose of 2000 mg/kg body weight of test item during the experimental period No local signs of toxicity (e.g.: Abrasion, Alopecia, Erythema, Oedema, Scale Formation etc.) were observed at limit dose of 2
- Gross pathology:
- The external and internal gross pathological observation of all terminally sacrificed animals did not show any pathological abnormality
- Other findings:
- No data available
Any other information on results incl. tables
Individual Animal Mortality Record
Animal No. |
Sex |
Days of Observation (0 to 14) |
|
Morning Observations |
Evening Observations |
||
01 |
Male |
No mortality and morbidity |
No mortality and morbidity |
02 |
No mortality and morbidity |
No mortality and morbidity |
|
03 |
No mortality and morbidity |
No mortality and morbidity |
|
04 |
No mortality and morbidity |
No mortality and morbidity |
|
05 |
No mortality and morbidity |
No mortality and morbidity |
|
06 |
Female |
No mortality and morbidity |
No mortality and morbidity |
07 |
No mortality and morbidity |
No mortality and morbidity |
|
08 |
No mortality and morbidity |
No mortality and morbidity |
|
09 |
No mortality and morbidity |
No mortality and morbidity |
|
10 |
No mortality and morbidity |
No mortality and morbidity |
Summary of Animal Body Weight (g) and Body Weight Changes (%)
Sex |
Body Weight (gram) |
Body Weight Changes (%) |
||||
Day 0 |
Day 7 |
Day 14 |
Day 0-7 |
Day 0-14 |
||
Male |
Mean |
261.60 |
273.00 |
298.40 |
4.33 |
14.09 |
SD |
16.68 |
19.20 |
20.03 |
1.35 |
3.60 |
|
n |
5 |
5 |
5 |
5 |
5 |
|
Female |
Mean |
229.60 |
232.40 |
246.00 |
1.21 |
7.14 |
SD |
7.13 |
8.02 |
8.92 |
0.70 |
1.45 |
|
n |
5 |
5 |
5 |
5 |
5 |
Gross Necropsy Observation
Animal No. |
Sex |
Gross Observation |
|
External |
Internal |
||
01 |
Male |
No abnormality detected |
No abnormality detected |
02 |
No abnormality detected |
No abnormality detected |
|
03 |
No abnormality detected |
No abnormality detected |
|
04 |
No abnormality detected |
No abnormality detected |
|
05 |
No abnormality detected |
No abnormality detected |
|
06 |
Female |
No abnormality detected |
No abnormality detected |
07 |
No abnormality detected |
No abnormality detected |
|
08 |
No abnormality detected |
No abnormality detected |
|
09 |
No abnormality detected |
No abnormality detected |
|
10 |
No abnormality detected |
No abnormality detected |
Applicant's summary and conclusion
- Interpretation of results:
- other: not classified
- Conclusions:
- The LD50 value was considered to be > 2000 mg/kg bw when Wistar male and female rats were treated with 2-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl] benzoxazole-5-sulphonamide.
- Executive summary:
In a acute dermal toxicity study, Wistar male and female rats treated with 2-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl] benzoxazole-5-sulphonamide in the concentration of 2000 mg/kg bw orally by gavage as per OECD 402 and observed for 14 days. No effect on survival, clinical sign and Local Signs/Skin Reactions were observed in treated male and female rats. Gain in body weight were observed on day 7 and 14, as compared to day 0 body weight, weighed prior to dosing in 2000 mg/kg bw treated male and female rats. In addition, No external and internal gross pathological abnormalities were observed in 2000 mg/kg bw treated male and female rats. Therefore, LD50 value was > 2000 mg/kg body weight when Wistar male and female rats treated with 2-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl] benzoxazole-5-sulphonamide orally by gavage.
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