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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Justification for type of information:
Data is from safety assessment report

Data source

Reference
Reference Type:
secondary source
Title:
Screening Assessment for the Challenge - Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-Bis(diethylamino)- (Solvent Red 49)
Author:
Environment Canada (EC) Health Canada (HC)
Year:
2010
Bibliographic source:
Screening Assessment for the Challenge - Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-Bis(diethylamino)- (Solvent Red 49) Environment Canada (EC) Health Canada (HC) 2010

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
To evaluate the toxicity of 9-(2-carboxyphenyl)-3,6 bis(diethylamino)xanthylium chloride on Daphnia magna.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride
EC Number:
201-383-9
EC Name:
9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride
Cas Number:
81-88-9
Molecular formula:
C28H31N2O3.Cl
IUPAC Name:
9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthenium chloride
Details on test material:
Name - 9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride
Common Name - Rhodamine B
InChI - 1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)
14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H
Smiles -1(c2c(oc3c1ccc(\c3)=[N+](/CC)CC)cc(N(CC)CC)cc2)c1c(cccc1)C(O)=O.[ClH-]
Molecular formula - C28H31ClN2O3
Molecular weight - 479.017 g/mole
Specific details on test material used for the study:
Name - 9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride
Common Name - Rhodamine B
InChI - 1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)
14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H
Smiles -1(c2c(oc3c1ccc(\c3)=[N+](/CC)CC)cc(N(CC)CC)cc2)c1c(cccc1)C(O)=O.[ClH-]
Molecular formula - C28H31ClN2O3
Molecular weight - 479.017 g/mole

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
No data available

Test solutions

Vehicle:
not specified
Details on test solutions:
No data available

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h

Results and discussion

Effect concentrations
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
22 mg/L
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The effect concentration EC50 was observed to be 22mg/l for 9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride after 24h.
Executive summary:

9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride was used as a test material to evaluate its toxicity for Daphnia magna The effect concentration EC50 was observed to be 22mg/l for 9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride after 24h.