Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Justification for type of information:
Data is from safety assessment report

Data source

Reference
Reference Type:
secondary source
Title:
Screening Assessment for the Challenge - Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-Bis(diethylamino)- (Solvent Red 49)
Author:
Environment Canada (EC) Health Canada (HC)
Year:
2010
Bibliographic source:
Screening Assessment for the Challenge - Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-Bis(diethylamino)- (Solvent Red 49) Environment Canada (EC) Health Canada (HC) 2010

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
To evaluate the toxicity of 9-(2-carboxyphenyl)-3,6 bis(diethylamino)xanthylium chloride on Daphnia magna.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride
EC Number:
201-383-9
EC Name:
9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride
Cas Number:
81-88-9
Molecular formula:
C28H31N2O3.Cl
IUPAC Name:
9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthenium chloride
Details on test material:
Name - 9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride
Common Name - Rhodamine B
InChI - 1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)
14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H
Smiles -1(c2c(oc3c1ccc(\c3)=[N+](/CC)CC)cc(N(CC)CC)cc2)c1c(cccc1)C(O)=O.[ClH-]
Molecular formula - C28H31ClN2O3
Molecular weight - 479.017 g/mole
Specific details on test material used for the study:
Name - 9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride
Common Name - Rhodamine B
InChI - 1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)
14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H
Smiles -1(c2c(oc3c1ccc(\c3)=[N+](/CC)CC)cc(N(CC)CC)cc2)c1c(cccc1)C(O)=O.[ClH-]
Molecular formula - C28H31ClN2O3
Molecular weight - 479.017 g/mole

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
No data available

Test solutions

Vehicle:
not specified
Details on test solutions:
No data available

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h

Results and discussion

Effect concentrations
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
22 mg/L
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The effect concentration EC50 was observed to be 22mg/l for 9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride after 24h.
Executive summary:

9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride was used as a test material to evaluate its toxicity for Daphnia magna The effect concentration EC50 was observed to be 22mg/l for 9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride after 24h.