2-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl]-1,3-dimethyl-1H-benzimidazolium chloride

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for 2-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl]-1, 3-dimethyl-1H-benzimidazolium chloride

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl]-1,3-dimethyl-1H-benzimidazolium chloride

EC Number:

249-694-9

EC Name:

2-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl]-1,3-dimethyl-1H-benzimidazolium chloride

Cas Number:

29556-33-0

Molecular formula:

C22H24N3O2.Cl

IUPAC Name:

2-[7-(diethylamino)-2-oxo-2H-chromen-3-yl]-1,3-dimethyl-2,3-dihydro-1H-3,1-benzimidazol-1-ium chloride

Test material form:

solid: particulate/powder

Details on test material:

- Name of test material: 2-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl]-1,3-dimethyl-1H-benzimidazolium chloride
- Molecular formula: C22H24N3O2.Cl
- Molecular weight: 347.4159 g/mol
- Smiles notation (if other than submission substance): c1(c2c(oc3c(c2)ccc(N(CC)CC)c3)=O)nc2ccccc2n1C
- InChl (if other than submission substance): 1S/C21H21N3O2/c1-4-24(5-2)15-11-10-14-12-16(21(25)26-19(14)13-15)20-22-17-8-6-7-9-18(17)23(20)3/h6-13H,4-5H2,1-3H3
- Substance type: Organic
- Physical state: Bright Yellow to Greenish Yellow Powder

Specific details on test material used for the study:

- Name of test material: 2-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl]-1,3-dimethyl-1H-benzimidazolium chloride
- Molecular formula: C22H24N3O2.Cl
- Molecular weight: 347.4159 g/mol
- Smiles notation (if other than submission substance): c1(c2c(oc3c(c2)ccc(N(CC)CC)c3)=O)nc2ccccc2n1C
- InChl (if other than submission substance): 1S/C21H21N3O2/c1-4-24(5-2)15-11-10-14-12-16(21(25)26-19(14)13-15)20-22-17-8-6-7-9-18(17)23(20)3/h6-13H,4-5H2,1-3H3
- Substance type: Organic
- Physical state: Bright Yellow to Greenish Yellow Powder
-Batch No.: FG/15-16/2453

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Details on test solutions:

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD):average 3.42 cm
- Weight at study initiation (mean and range, SD): average 0.335 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 12 hours light and 12 hours darkness
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

148.5 mg of CaCO3

Test temperature:

28°C

pH:

7.1

Dissolved oxygen:

7.4 mg/L

Nominal and measured concentrations:

100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: plastic aquaria
- volume: 2 liters
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod:12 hours light and 12-hour darkness

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

8.1. Mortality and symptoms:

Nominal Concentration (mg/l)	Number of fish		Mortality (cumulated) after
			3h		6h		24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	-	-	-	-	-	-	-	-	-	-	-	-
100	4	4	-	-	-	-	-	-	-	-	-	-	-	-

Visible Symptoms:

Nominal Concentration (mg/l)

Number of fish

symptom (cumulated) after

3h

6h

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

100

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

pH and oxygen content:

Nominal Concentration (mg/l)	Number of fish		pH readings after
			24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	8.21	8.22	7.57	7.60	7.40	7.47	7.51	7.53
100	4	4	7.65	7.64	7.40	8.21	8.21	8.17	8.11	8.13

Nominal Concentration (mg/l)	Number of fish		Temperature (°C) after
			24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	21	21	25	25	24	24	23	23
100	4	4	21	21	25	25	24	24	23	23

Applicant's summary and conclusion

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. 29556-33-0on Zebra Fish Danio rerio was found to be >100mg/L.

Executive summary:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for 2-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl]-1 ,3-dimethyl-1H-benzimidazolium chloride . The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L.

The nominal concentration selected for the experiment was 100 mg/l and Zebra fish were exposed to this concentration for 96 hours. The lethal concentrations LC50 was found to be > 100mg/L.

On the basis of above effect concentration 2-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl]-1 ,3-dimethyl-1H-benzimidazolium chloride is not considered as toxic to fish.