2-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl]-1,3-dimethyl-1H-benzimidazolium chloride

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from J-CHECK and Envichem authoritative database

Data source

Reference

Reference 1

Reference Type:

other: authoritative database

Title:

Bioaccumulation: aquatic/sediment

Author:

National Institute of Technology and Evaluation.

Year:

2017

Bibliographic source:

Japan chemicals collaborative knowledge database (J-check), 2017

Reference 2

Reference Type:

other: authoritative database

Title:

Bioaccumulation: aquatic/sediment

Author:

Data bank of Environmental Properties of Chemicals (EnviChem)

Year:

2014

Bibliographic source:

Data bank of Environmental Properties of Chemicals (EnviChem), 2014.

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances"

Principles of method if other than guideline:

Bioaccumulation study was conducted on test organism Cyprinus carpio for 8 weeks for evaluating the bioconcentration factor (BCF value) of substance N, N-diethylaniline. The study was performed according to other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances".

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

N,N-diethylaniline

EC Number:

202-088-8

EC Name:

N,N-diethylaniline

Cas Number:

91-66-7

Molecular formula:

C10H15N

IUPAC Name:

N, N-diethylaniline

Test material form:

liquid

Specific details on test material used for the study:

- Name of test material: N, N-diethylaniline
- Molecular formula: C10H15N
- Molecular weight: 149.236 g/mol
- Smiles notation: c1(N(CC)CC)ccccc1
- InChl: 1S/C10H15N/c1-3-11(4-2)10-8-6-5-7-9-10/h5-9H,3-4H2,1-2H3
- Substance type: Organic
- Physical state: Liquid
- Water solubility: slightly soluble

Radiolabelling:

not specified

Test solutions

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Chemical name of vehicle: HCO-40, 1st Concentration area: 1.00 ppm, 2nd Concentration area: 0.1 ppm

Test organisms

Test organisms (species):

Cyprinus carpio

Study design

Route of exposure:

aqueous

Test type:

static

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

8 wk

Test conditions

Details on test conditions:

RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations: TLm (48 hr) 16.8 ppm (16.8 mg/l) (w/v), Rice fish (Oryzias latipes)

Nominal and measured concentrations:

Nominal concentrations:
1st concentration area: 0.2 ppm (0.2 mg/l) (w/v)
2nd concentration area: 0.02 ppm (0.02 mg/l) (w/v)

Reference substance (positive control):

not specified

Results and discussion

Bioaccumulation factor

Bioaccumulation factor 1

Conc. / dose:

0.2 mg/L

Type:

BCF

Value:

44 L/kg

Basis:

whole body w.w.

Time of plateau:

8 wk

Remarks on result:

other: Other details not known

Bioaccumulation factor 2

Conc. / dose:

0.2 mg/L

Type:

BCF

Value:

161 L/kg

Basis:

whole body w.w.

Time of plateau:

8 wk

Remarks on result:

other: Other details not known

Bioaccumulation factor 3

Conc. / dose:

0.02 mg/L

Type:

BCF

Value:

17 L/kg

Basis:

whole body w.w.

Time of plateau:

8 wk

Remarks on result:

other: Other details not known

Bioaccumulation factor 4

Conc. / dose:

0.02 mg/L

Type:

BCF

Value:

125 L/kg

Basis:

whole body w.w.

Time of plateau:

8 wk

Remarks on result:

other: Other details not known

Any other information on results incl. tables

Bioaccumulation test results:

 

		2w	3w	4w	6w
1st Concentration area	Test substance concentration (ppm)	0.166	0.157	0.161	0.162
	Bioconcentration factor (L/kg)	75	95	44	161
		63	118	57	64
2nd Concentration area	Test substance concentration (ppm)	0.021	0.020	0.019	0.020
	Bioconcentration factor (L/kg)	98	57	28	125
		80	17	29	75

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The bioconcentration factor (BCF value) of substance N, N-diethylaniline on Cyprinus carpio was determined to be 44 and 161 L/Kg at a conc. of 0.2 mg/l and 17 and 125 L/Kg at a conc. of 0.02 mg/l, respectively.

Executive summary:

Bioaccumulation study was conducted on test organism Cyprinus carpio for 8 weeks for evaluating the bioconcentration factor (BCF value) of substance N, N-diethylaniline (CAS no. 91-66-7) under static conditions.The study was performed according to other guideline"Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances". Cyprinus carpio was used as a test organism for the study. HCO-40 was used as a vehicle. Test chemical nominal conc. used for the study were 0.2 mg/l and 0.02 mg/l (w/v), respectively. Range finding study involve the TLm (48 hr) of 16.8 ppm (16.8 mg/l) (w/v) on Rice fish (Oryzias latipes). The bioconcentration factor (BCF value) of substance N, N-diethylaniline on Cyprinus carpio was determined to be 44 and 161 L/Kg at a conc. of 0.2 mg/l and 17 and 125 L/Kg at a conc. of 0.02 mg/l, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the chemical N, N-diethylaniline is not expected to bioaccumulate in the food chain.