Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
May/June 2015
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study conducted according to OECD guideline and Good Laboratory Practice. The results in this study are consistent with all validity criteria and the test is valid according to the test guideline of this study.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report date:
2015

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
sra-[(2E)-2-butenedioato(2-)hydroxy aluminium]
EC Number:
810-213-8
Cas Number:
1370461-06-5
Molecular formula:
C4 H3 Al O5
IUPAC Name:
sra-[(2E)-2-butenedioato(2-)hydroxy aluminium]
Test material form:
solid: crystalline
Details on test material:
Synonym: Basolite A520
Batch identification: 13-0001-2
Content: 85.8 g/100 g (based on the determined content of fumarate)

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
Municipal activated sludge from the wastewater treatment plant of of Mannheim, Germany.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
20 mg/L
Based on:
other: Total Organic Carbon
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
Test temperature: 22 °C +/-2
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
ca. 55
Sampling time:
28 d
Details on results:
Duration of the adaptation phase: 4 days

BOD5 / COD results

Results with reference substance:
Degree of biodegradation after 14 days: 67% CO2/ThCO2

Any other information on results incl. tables

The selected concentration of 20 mg/L TOC/L (approx. 74 mg/L test substance), tested in an additional inhibition control, caused no toxic effect to microorganisms.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
other:
Conclusions:
The test substance was not readily biodegradable under the conditions of this test. Based on the rate of biodegradation of 50-60% CO2/ThCO2 at the end of the test the test substance can be evaluated as moderately or partly biodegradable in this test.