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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1967
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1967
Report date:
1967

Materials and methods

Test guidelineopen allclose all
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Qualifier:
according to guideline
Guideline:
other: Federal Hazardous Substances Labeling Act (1964)
Deviations:
no
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Diethyl [[3,5-bis(1,1-dimethylethyl)-4-hydroxyphenyl]methyl]phosphonate
EC Number:
213-551-9
EC Name:
Diethyl [[3,5-bis(1,1-dimethylethyl)-4-hydroxyphenyl]methyl]phosphonate
Cas Number:
976-56-7
Molecular formula:
C19H33O4P
IUPAC Name:
diethyl [(3,5-di-tert-butyl-4-hydroxyphenyl)methyl]phosphonate

Test animals / tissue source

Species:
rabbit
Strain:
not specified

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not required
Amount / concentration applied:
TEST MATERIAL
- Amount applied: 100 mg
Duration of treatment / exposure:
directly after application, the eyelids were held together for a second and eyes were not washed out afterwards
Observation period (in vivo):
72 h
Number of animals or in vitro replicates:
6 (3 males, 3 females)
Details on study design:
SCORING SYSTEM:
according to "Illustrated Guide for Grading Eye Irritation by Hazardous Substances", issued by the Superintendent of Documents, Govemment Printing Office, Washington, D.C.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 24 h /48 h / 72 h
Score:
0
Max. score:
4
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 24 h /48 h / 72 h
Score:
0.05
Max. score:
2
Reversibility:
fully reversible within: 48 h
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 24 h /48 h / 72 h
Score:
< 0.16
Max. score:
3
Reversibility:
fully reversible within: 48 h
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 24 h /48 h / 72 h
Score:
< 0.11
Max. score:
4
Reversibility:
fully reversible within: 48 h
Irritant / corrosive response data:
After 24 hours, minimal redness and chemosis were observed in three and two animals, respectively. In addition, one animal showed minimal reaction of the iris with a score of 1 after 24 hours. All findings cleared after 48 hours.

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met