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Environmental fate & pathways

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Biodegradation in water

A modified sturm test was conducted to asses the ready biodegradability of the test substance. The study was conducted according to EU guideline C.5 of 1984 (84/449/EEC), except for the following deviations: The volume of the test solution was reduced from 3.0 L to 1.5 L. The CO2 formed by biodegradation was absorbed with NaOH and determined on a carbon analyzer. Due to the poor solubility of the test substance in water, an emulsifier was used to achieve a better distribution in the medium. The test substance was added to the medium, which was then homogenized with Tween 80. As inoculum, bacteria collected from activated sludge of a sewage treatment plant were used. The test substance was tested in 2 concentrations: 10.8 mg/L and 21 mg/L. The duration of the test was 28 days, CO2 evolution was measured on days 5, 9, 13, 16, 19, 23, 27 and 29. Under the test conditions chosen the test substance was moderately/partly biodegradable (27 % degredation after 28 days).