Sodium dicyanoamide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

05 June 2000 - 24 July 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to OECD Guideline 203 and GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Sodium dicyanoamide

EC Number:

217-703-5

EC Name:

Sodium dicyanoamide

Cas Number:

1934-75-4

Molecular formula:

C2HN3.Na

IUPAC Name:

sodium dicyanoazanide

Constituent 2

Reference substance name:

Sodiumdicyanamide

IUPAC Name:

Sodiumdicyanamide

Test material form:

other: solid

Details on test material:

Appearance: solid, white
Purity: 98.1%
Lot-No.: 62/3727
Storage: RT
Expiry date: January 2002

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

400 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

160 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

As a result of the supporting analyses, it could be shown that the test substance was sufficiently (103% recovery on the basis of t₀) stable during the period of incubation. Thus, the inhibition values obtained within this study are based on the nominal concentrations.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The concentration where no toxic effect occured (NOEC after 96h) was calculated as being 160 mg/L test substance. The LC50 (96h) was calculated as 400 mg/L.

Executive summary:

The study was performed in year 2000 according to OECD-Test Guideline 203 and GLP. In order to investigate the influence of the test substance towards the fish, the animals were exposed to different concentrations of the test substance for 96h. Daily the test solutions were examined for dead animals.The concentration where no toxic effect occured (NOEC after 96h) was calculated as being 160 mg/L test substance. The LC50 (96h) was calculated as 400 mg/L. The concentration where all test animals died (LC100) was calculated as 1000 mg/L.