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Physical & Chemical properties

Water solubility

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Description of key information

The water solubility of Z-Acetate at 25°C was determined to be 3.5 µg/L using a modified column elution test method.

Key value for chemical safety assessment

Additional information

The water solubility of Z-Acetate was determined using a modified column elution test similar to OECD guideline 105. Triplicate test systems were prepared and incubated for 0, 1, 2, 5, 6, 7, 8, 9, 12, 13 and 14 day at 25°C ± 1 °C. Triplicate aliquots of water samples were taken on each sampling day, diluted with acetonitrile and analyzed using LC/MS/MS. Additionally, the syringe was rinsed with acetonitrile and the rinseate analyzed for any syringe-bound Z-Acetate. The total Z-Acetate in the water sample and the syringe rinseate was combined as total concentration of Z-Acetate in the water sample. A water saturation equilibrium was reached by day 5. The overall average water solubility concentration for Z-Acetate was 3.50 µg/L, based on results for all three test system from day 5 through day 14 (n = 72).The overall RSD% was 50%.

This is a modified guideline study conducted under GLP. The modification was made to accommodate the liquid state of the test substance whereas a flow through cell containing the liquid substance was used in place of the flow through column design. The modification of test method does not affect the outcome of the test results. The precision value of RSD of 50% was due to the measurement of very low level concentrations, and near the limits of quantitation for the method. However, this does not affect the conclusion that the test substance has very low water solubility. Therefore, this study is considered as a key study, valid with restriction with a Klimisch 2 ranking. A water solubility description of “negligible” was also found. However, due to few details available, the reliability of the description is not assignable.