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Diss Factsheets

Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2006-09-29 to 2007-04-18
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
GLP study performed according to OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC)) and EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC)) without deviations.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report date:
2007

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Remarks:
The testing facility indicated that the protocol was followed without deviation.
Qualifier:
according to guideline
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Remarks:
The testing facility indicated that the protocol was followed without deviation.
Principles of method if other than guideline:
Not applicable
GLP compliance:
yes (incl. QA statement)
Remarks:
: Certificate issued by the United Kingdom GLP Monitoring Authorities
Type of method:
HPLC estimation method
Media:
soil

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): VRT-753136
- Molecular formula (if other than submission substance): not applicable
- Molecular weight (if other than submission substance): not applicable
- Smiles notation (if other than submission substance): not applicable
- InChl (if other than submission substance): not applicable
- Structural formula attached as image file (if other than submission substance): not applicable
- Substance type: no data
- Physical state: white powder
- Analytical purity: 99.4% w/w by High Performance Liquid Chromatography (HPLC)
- Impurities (identity and concentrations): individual unknown components were detected at 0.03 area%; isopropanol (residual solvent) at 300 ppm
- Composition of test material, percentage of components: not applicable
- Isomers composition: not applicable
- Purity test date: 2006-05-25
- Lot/batch No.: 25515
- Expiration date of the lot/batch: May 2008
- Stability under test conditions: no data
- Storage condition of test material: at room temperature
- Other: no data
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Radiolabelling:
no

Study design

Test temperature:
25 deg C

HPLC method

Details on study design: HPLC method:
EQUIPMENT
- Apparatus:
- Type: Hewlett Packard 1050 Liquid Chromatograph
- Type, material and dimension of analytical (guard) column: Hypersil CPS (25 cm x 4.6 mm internal diameter)
- Detection system: UV set at 210 nm


MOBILE PHASES
- Type: acetonitrile:buffer solution (55:45 v/v), where phosphate and borate buffer solutions were employed for the pH 4 and pH 10 tests respectively
- Experiments with additives carried out on separate columns: no
- pH: 4-10
- Solutes for dissolving test and reference substances: no


DETERMINATION OF DEAD TIME
- Method: by inert substances (formamide) which is not retained by the column


REFERENCE SUBSTANCES
- Identity: acetanilide; nitrobenzene; naphthalene; 2-methylnaphthalene; anthracene; DDT


DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 20 uL (78.0 mg/L in HPLC mobile phase), containing internal deadtime standard (formamide) at 844 mg/L
- Quantity of reference substances (mg/L): acetanilide (51.0); nitrobenzene (60.3); naphthalene (50.0); 2-methylnaphthalene (53.1); anthracene (99.6); DDT (102)
- Intervals of calibration: no data


REPETITIONS
- Number of determinations: 2


EVALUATION
- Calculation of capacity factors k': k' = (Tr - To)/To, where Tr is the measured retention time of sample or reference and To is the retention time of the internal deadtime standard.
- Calculation of retention times: The deadtime was subtracted from the experimental retention time for the test substance and the average value of the replicate runs was calculated at both pH 4.0 and 10.0.
- Determination of the log Koc value: Known log Koc values were used for the reference substances with values between 1.3 and 5.6. The adsorption coefficient was deduced from the capacity factor (k').

Batch equilibrium or other method

Analytical monitoring:
not required
Details on sampling:
Not applicable
Details on matrix:
Not applicable
Details on test conditions:
Not applicable
Computational methods:
Not applicable

Results and discussion

Results: HPLC method

Details on results (HPLC method):
- Retention times of reference substances used for calibration: 3.6-7.4 min (pH 4) and 3.4-5.5 (pH 10)
- Details of fitted regression line (log k' vs. log Koc): log Koc = 5.19 * Log k + 4.56 (pH 4)
- Average retention data for test substance: 3.7 min at pH 4.0 and 3.3 at pH 10.0, Log Koc values for the test substance were 1.4 (pH 4) and <1.3 (pH 10) (less than the lowest available reference material (acetanilide)), respectively.
- At pH 10, no regression line was constructed as the retention time of the test substance was less than the retention time of the lowest available reference material

Results: Batch equilibrium or other method

Adsorption and desorption constants:
Not applicable
Recovery of test material:
Not applicable
Concentration of test substance at end of adsorption equilibration period:
Not applicable
Concentration of test substance at end of desorption equilibration period:
Not applicable
Details on results (Batch equilibrium method):
Not applicable
Statistics:
Not applicable

Any other information on results incl. tables

Not applicable

Applicant's summary and conclusion

Conclusions:
The test substance was found to be have logKoc values of 1.4 and less than 1.3 at pH 4 and pH 10 respectively.
Executive summary:

Not applicable