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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented study according to international accepted guideline and GLP compliant.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report Date:
2015

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Details on test material:
Test item: 4-Chloro-4’-fluorobutyrophenoneCAS number: 3874-54-2EC number: 223-395-3Molecular formula: C10H10ClFOMolecular weight: 200.64 g/molBatch number STBD6247VPhysical state: liquidStorage: cool, dry place in a tightly closed container

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
Type of inoculum: aerobic mixed bacterial cultureSource: Municipal wastewater treatment plant, Esztergom-Kertváros
Duration of test (contact time):
28 d
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
PREPARATION OF INOCULUM TEST CULTUREBefore preparation of inoculum test culture, the dry substance content of the stock culture was determined. The measured value was 7.3 g/L. 250.0 ml inoculum test culture with 1.0 g/L dry substance was made by mixing 34 ml of stock culture with 216 ml mineral medium.PREPARATION OF REFERENCE SOLUTIONFor the reference solution 320.13 mg sodium acetate was diluted in 100 ml mineral medium.PREPARATION OF STOCK SOLUTION OF TEST ITEMFor the stock solution 150.06 mg test item was diluted in 1000 ml mineral medium. A homogenous emulsion was prepared by stirring.PREPARATION OF TEST VESSELSFollowing test vessels were prepared and analysed for the study:- Test item (in two replicates)- Blank control (in two replicates)- Reference solution (positive control)- Reference and test item solution (toxicity control)- Sterilized solution of test item (abiotic control)
Reference substance
Reference substance:
acetic acid, sodium salt

Results and discussion

% Degradation
Parameter:
% degradation (O2 consumption)
Value:
18
Sampling time:
28 d
Remarks on result:
other: mean of two replicates

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
other: not readily biodegradable
Conclusions:
According to OECD guideline the test item should be classified as readily biodegradable if the percentage biodegradation reaches 60% of theoretical oxygen demand within 28 day period in a 10-day window. Based on ready biodegradability testing results the test item 4-Chloro-4’-fluorobutyrophenone is not readily biodegradable.