Pregn-4-ene-3,20-dione, 21-(acetyloxy)-6-fluoro-16-methyl-, (6.alpha.,16.alpha.)-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Oct 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to OECD guideline under GLP

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Principles of method if other than guideline:

Not relevant

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

21-Acetoxy-6 alpha-fluoro-16 alpha-methyl-4-pregnene-3,20-dione

EC Number:

603-033-4

Cas Number:

1251-28-1

Molecular formula:

C24 H33 F O4

IUPAC Name:

21-Acetoxy-6 alpha-fluoro-16 alpha-methyl-4-pregnene-3,20-dione

Details on test material:

- Name of test material (as cited in study report): Alpha-FM-DOCA
- Analytical Purity: 71%
- Lot/batch No.: 21602001

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 5.3 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

A saturated solution of Alpha-FM-DOCA had no effect on the mobility of Daphnia. It can be concluded that the EC50/48 hours of the test substance is higher than a saturated solution.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

A saturated solution (5.3 mg/L) of the test compound did not produce immobilization in Daphnia magna.
The EC5048 hours to Daphnia magna is higher than a saturated solution .

Executive summary:

The purpose of this study was to determine the acute toxicity (immobilization, EC50/48 hours) of Alpha-FM-DOCA (ZK 4841) with Daphnia magna in order to evaluate the aquatic toxicity.

20 juvenile Daphnia magna were used for the test solution and 20 for the tap water control. The Daphnia were exposed for a period of 48 hours under static conditions. Immobilization was recorded at 24 and 48 hours. For the preparation of the test solution, a suspension of 100 mg/L was stirred for 24 hours. This suspension was filtered thraugh a glassfibre filter. The saturated solution obtained by this procedure was used. The organic carbon concentration of the test solution was analysed with a TOC analyser at the start of exposure and 48 hours thereafter. The substance concentration was calculated based on the molecular formula. The concentration was 5.3 mg/L.

No immobilized Daphnia were observed in the test or in the control solution.