Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 283-626-9 | CAS number: 84696-07-1 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Cupressus sempervirens, Pinaceae.
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
An acute aquatic toxicity study was performed on Daphnia magna in accordance with GLP and OECD Guideline No 202 and WAF conditions.
Under the test conditions, the 48h-EC50 of CYPRESS OIL on Daphnia magna was 3.0 mg/L, based on mean measured concentrations.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 3 mg/L
Additional information
An acute aquatic toxicity study was performed on Daphnia magna in accordance with GLP and OECD Guideline No 202 and WAF conditions. Twenty daphnids (four replicates, five daphnids per replicate) were exposed to an aqueous solution of Cypress oil at the required nominal test concentrations of 1.5, 1.9, 2.4, 3.1, 3.9 and 5.0 mg test item.L-1. Immobilisation of daphnids was determined in a semi-static 48-h test by visual observation after 24 and 48 h.
The concentrations of the test item were measured at the start (t = 0 h), at t = 24 h (new and old solutions) and at the end of the test (t = 48 h). Sufficient analytical evidence is provided that every effort was made to maintain the WAFs concentrations. However, initial measured concentrations were lower compared to their nominal values (loading rates). The analytical results showed that WAFs were enough stable over the study period (overall within or close to the ± 20% of the initial concentration). However, a WAF is by definition for complex mixtures for which individual concentration of each constituent differs dur to own properties. For this reason it was consider acceptable to base the results on nominal loading values. At 24h, immobilisaiton was at 0% 1.5, 1.9, 2.4, 3.1 mg test item.L-1 (loading), 5% at 3.9mg/L (loading) and 90% at 5.0mg/L (loading).
At 48h, immobilization was 0% at 1.5, 1.9, 2.4 mg test item.L-1 (loading), 75% at 3.1 mg test item.L-1 and 100% at 3.9 and 5.0 mg test item.L-1 (loading).
Therefore, the 48h-EC50of Cypress oil for Daphnia magna was estimated to be 3.0 mg/L.
Based on the results of this study, Cypress oil would not be classified as acute 1 to aquatic organisms in accordance with the classification of the CLP.
This toxicity study is acceptable and can be used for that endpoint.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
