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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
None
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Principles of method if other than guideline:
None
GLP compliance:
yes
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):None
Analytical monitoring:
not specified
Details on sampling:
None
Vehicle:
yes
Details on test solutions:
Identity and concentration of auxiliary solvent for dispersal: Vorlösung in 1 % v/v Tween 80-Dimethylformamid
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM- Common name: Daphnia magna Straus.- Source: Laboratory culture originating from a strain supplied by the Institute National de recherche Chimique Appliquee (I.R.Ch.A.), France- Age at study initiation (mean and range, SD): young daphnids produced overnight were used.
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Post exposure observation period:
None
Hardness:
50 mg CaCO3/L
Test temperature:
no data
pH:
no data
Dissolved oxygen:
no data
Salinity:
not applicable
Nominal and measured concentrations:
% Concentration loss over test: 0.010, 0.10, 0.50 and 1.0 mg/l
Details on test conditions:
TEST SYSTEM- Test vessel: Glass jars- Type (delete if not applicable): open- Material, size, headspace, fill volume: 250 ml test solution- Aeration: None- Renewal rate of test solution (frequency/flow rate): None- No. of organisms per vessel: 0.5mg/l, four replicates- No. of vessels per concentration (replicates): 0.010, 0.10, 0.50 and 1.0 mg/l- Loading: 25 ml test solution per organismOTHER TEST CONDITIONS- Photoperiod: 16h light : 8h dark
Reference substance (positive control):
not specified
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 0.5 mg/L
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 0.5 mg/L
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 0.5 mg/L
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
>= 0.5 mg/L
Details on results:
None
Results with reference substance (positive control):
None
Reported statistics and error estimates:
None

Cumulative Immobilisation Data for Daphnia magna exposed for 48 hours to D-523:

Nominal Conc. Of test material (mg/l) Cumulative immobilised Daphnia                                                (initial population : 10 per replicate)
24 hours 48 hours
No. per replicate Total % No. per replicate Total %
Control                           R1 0 0 0 0 0 0
                                          R2 0 0
Solvent Control          R1 0 0 0 0 0 0
                                          R2 0 0
0.5                                    R1 0 0 0 0 0 0
                                         R2 0 0
                                         R3 0 0
                                         R4 0 0

R1 - R4 = Replicates 1 -4

Validity criteria fulfilled:
not specified
Conclusions:
The NOEC and EC50 value after 48 hours were determined to be greater than 0.50 mg/l.
Executive summary:

FAT 40554 was tested for its acute toxicity to Daphnia magna in a 48 hours static test. The study was performed under Good Laboratory Practice (GLP) conditions in Switzerland.

The experiment was done according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adopted 4th April 1984, (issued 1993) and EU-Guideline 92/69, EEC Nr. L 383 A, C. 2, (29th Dec. 1992).

The nominal concentrations tested were0.010, 0.10, 0.50 and 1.0 mg/l test article, a vehicle and a blank control without any additions. 0.50mg/l was the highest test concentration that could be achieved due to the limited solubility of the test material in water and auxiliary solvent and having due regard to the amount of auxiliary solvent permitted in the test under the OECD Guidelines.

No immobilization and abnormal behaviour of the Daphnia was observed in the controls. The NOEC and EC0 value after 48 hours were determined to be 39 mg/l, the EC100 value are > 203 mg/l. The EC50 (48 hrs) value was found to be greater than 0.50 mg/l

According to Directive 93/21/EEC (adaptation to Directive 67/548/EEC) the ecotoxicological classification of FAT 40554 is not toxic to Daphnia magna after 48 hours.

Description of key information

The EC50 (48 hrs) value for FAT 4055411 in daphnia magna was found to be greater than 0.50 mg/l.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
0.5 mg/L

Additional information

In a key study, FAT 40554 was tested for its acute toxicity to Daphnia magna in a 48 hours static test. The study was performed under Good Laboratory Practice (GLP) conditions in Switzerland.

The experiment was done according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adopted 4th April 1984, (issued 1993) and EU-Guideline 92/69, EEC Nr. L 383 A, C. 2, (29th Dec. 1992).

The nominal concentrations tested were0.010, 0.10, 0.50 and 1.0 mg/l test article, a vehicle and a blank control without any additions. 0.50mg/l was the highest test concentration that could be achieved due to the limited solubility of the test material in water and auxiliary solvent and having due regard to the amount of auxiliary solvent permitted in the test under the OECD Guidelines.

No immobilization and abnormal behaviour of the Daphnia was observed in the controls. The NOEC and EC0 value after 48 hours were determined to be 39 mg/l, the EC100 value are > 203 mg/l. The EC50 (48 hrs) value was found to be greater than 0.50 mg/l

According to Directive 93/21/EEC (adaptation to Directive 67/548/EEC) the ecotoxicological classification of FAT 40554 is not toxic to Daphnia magna after 48 hours.