Hydrocarbons, C11-C13 (odd numbered), n-alkanes, <2% aromatics

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

1986

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication which meets basic scientific principles. Experimental data for C11 component of Hydrocarbons, C11 -C13 (odd numbered), n-alkanes.

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Principles of method if other than guideline:

48h acute toxicity test with Daphnia magna

GLP compliance:

no

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 1000 mg/L loading rate WSF
- Sampling method: 100 mL aliquots were in the same prepared as the test solutions for the test.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution of 1000 mg test substance/L was stirred for 24 hours and the resulting solution was allowed to stand for 24 hrs. The
aqueous solution used for testing was then drained off through a stopcock at the bottom of the vessel. Thus, the WSF of a 1000 mg/L loading was tested. In addition 100 mL aliquots were in the same way run into glass-stoppered 100 mL bottles for test substance analysis.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: in-house culture
- Age at study initiation (mean and range, SD): < 24h
- Feeding during test
- Food type: no

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

210 mg/L as CaCO3

Test temperature:

20 °C

pH:

7.5 - 8.3

Dissolved oxygen:

> 6.5 mg/L

Nominal and measured concentrations:

Nominal: 1000 mg/L loading rate WAF
Measured (initial): < 0.05 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL conical flasksclosed with glass-stoppers and almost completely filled with test solution
- No. of organisms per vessel: 25
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): not specified

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic medium prepared by addition of several salts to groundwater from a locality near Linschoten (The Netherlands): 1.19 mmol/L Na+, 0.2 mmol/L K+, 1.36 mmol/L Ca2+, 0.73 mmol/L Mg2+, 2.72 mmol/L Cl-, 0.73 mmol/L SO42-, 1.39 mmol/L HCO3-. The groundwater contains several other trace elements (<< 1 mg/L) .
- Intervals of water quality measurement: Oxygen and pH were monitored.

EFFECT PARAMETERS MEASURED: Immobility was recorded after 48h test duration.

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

loading rate WSF

Basis for effect:

mobility

Remarks on result:

other: n-undecane: Daphnia magna

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 0.05 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

dissolved

Remarks:

loading rate WAF

Basis for effect:

mobility

Remarks on result:

other: n-undecane: Daphnia magna

Duration:

48 h

Dose descriptor:

LL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

loading rate

Basis for effect:

mortality

Remarks on result:

other: undecane and tridecane:

Details on results:

No toxic effects were observed within the range of solubility in water.

Validity criteria fulfilled:

not applicable

Conclusions:

The test substance is not toxic to Daphnia magna within the range of solubility in water.

Executive summary:

The acute toxicity of the test substance was tested in an 48h non-GLP study with Daphnia magna under static conditions. Due to its low water solubility a 1000 mg/L loading rate WSF was used in a limit test. The substance analytics using GC confirmed the low water solubility (< 0.05 mg/L). 25 daphnids were exposed over 48 h. No toxic effects were observed within the range of solubility in water.

Thus, the 48h-EL50 value is determined to be > 1000 mg/L based on the nominal and > 0.05 mg/L based on the initially measured test concentration.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Study period:

1986

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication which meets basic scientific principles. Experimental data for C11 component of Hydrocarbons, C11 -C13 (odd numbered), n-alkanes.

Qualifier:

no guideline followed

Qualifier:

equivalent or similar to guideline

Guideline:

EPA OPPTS 850.1020 (Gammarid Acute Toxicity Test)

Principles of method if other than guideline:

96h acute toxicity test with Chaetogammarus marinus

GLP compliance:

no

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 1000 mg/L loading rate WSF
- Sampling method: 100 mL aliquots were in the same prepared as the test solutions for the test.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution of 1000 mg test substance/L was stirred for 24 hours and the resulting solution was allowed to stand for 4 hrs. The
aqueous solution used for testing was then drained off through a stopcock at the bottom of the vessel. Thus, the WSF of a 1000 mg/L loading was tested. In addition 100 mL aliquots were in the same way run into glass-stoppered 100 mL bottles for test substance analysis.

Test organisms (species):

other aquatic crustacea: Chaetogammarus marinus

Details on test organisms:

TEST ORGANISM
- Source: in-house culture in seaweater
- Age at study initiation (mean and range, SD): young
- Length at study initiation: 5 mm
- Feeding during test
- Food type: Fucus spec.

Test type:

semi-static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

15 °C

pH:

7.5 - 8.3

Dissolved oxygen:

> 6.5 mg/L

Salinity:

2.8%

Nominal and measured concentrations:

Nominal: 1000 mg/L loading rate WAF
Measured (initial): < 0.05 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 mL closed scintillation vials almost completely filled with test solution
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Renewal rate of test solution (frequency/flow rate): Animals surviving after 24h were transferred to fresh test solutions prepared in the same way. This procedure was repeated to add up to a total duration of 96h.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural seawater
- Intervals of water quality measurement: Oxygen and pH were monitored.

EFFECT PARAMETERS MEASURED: Immobility was recorded after 96h test duration.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

EL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

loading rate WSF

Basis for effect:

mobility

Duration:

96 h

Dose descriptor:

EL50

Effect conc.:

> 0.05 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

dissolved

Remarks:

loading rate WAF

Basis for effect:

mobility

Details on results:

No toxic effects were observed within the range of solubility in water.

Validity criteria fulfilled:

not applicable

Conclusions:

The test substance is not toxic to Chaetogammarus marinus within the range of solubility in water.

Executive summary:

The acute toxicity of the test substance was tested in an 96h non-GLP study with Chaetogammarus marinus under semi-static conditions. Due to its low water solubility a 1000 mg/L loading rate WSF was used in a limit test. The substance analytics using GC confirmed the low water solubility (< 0.05 mg/L). 10 daphnids were exposed over 96 h. No toxic effects were observed within the range of solubility in water. Thus, the 96h-EL50 value is determined to be > 1000 mg/L based on the nominal and > 0.05 mg/L based on the initially measured test concentration.

Reference 3

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Study period:

1986

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication which meets basic scientific principles. Experimental data for C11 component of Hydrocarbons, C11 -C13 (odd numbered), n-alkanes.

Qualifier:

no guideline followed

Qualifier:

equivalent or similar to guideline

Guideline:

EPA OPPTS 850.1035 (Mysid Acute Toxicity Test)

Principles of method if other than guideline:

96h acute toxicity test with Mysidopsis bahia

GLP compliance:

no

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 1000 mg/L loading rate WSF
- Sampling method: 100 mL aliquots were in the same prepared as the test solutions for the test.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution of 1000 mg test substance/L was stirred for 24 hours and the resulting solution was allowed to stand for 4 hrs. The
aqueous solution used for testing was then drained off through a stopcock at the bottom of the vessel. Thus, the WSF of a 1000 mg/L loading was tested. In addition 100 mL aliquots were in the same way run into glass-stoppered 100 mL bottles for test substance analysis.

Test organisms (species):

Americamysis bahia (previous name: Mysidopsis bahia)

Details on test organisms:

TEST ORGANISM
- Source: in-house culture in seaweater
- Age at study initiation (mean and range, SD): 4 weeks
- Length at study initiation: 6 mm
- Feeding during test
- Food type: Artemia nauplii

Test type:

semi-static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

20 °C

pH:

7.5 - 8.3

Dissolved oxygen:

> 6.5 mg/L

Salinity:

2.8%

Nominal and measured concentrations:

Nominal: 1000 mg/L loading rate WAF
Measured (initial): < 0.05 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 mL closed scintillation vials almost completely filled with test solution
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Renewal rate of test solution (frequency/flow rate): Animals surviving after 24h were transferred to fresh test solutions prepared in the same way. This procedure was repeated to add up to a total duration of 96h.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural seawater
- Intervals of water quality measurement: Oxygen and pH were monitored.

EFFECT PARAMETERS MEASURED: Immobility was recorded after 96h test duration.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

EL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

loading rate WSF

Basis for effect:

mobility

Duration:

96 h

Dose descriptor:

EL50

Effect conc.:

> 0.05 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

dissolved

Remarks:

loading rate WAF

Basis for effect:

mobility

Details on results:

No toxic effects were observed within the range of solubility in water.

Validity criteria fulfilled:

not applicable

Conclusions:

The test substance is not toxic to Mysidopsis bahia within the range of solubility in water.

Executive summary:

The acute toxicity of the test substance was tested in an 96h non-GLP study with Mysidopsis bahia under semi-static conditions. Due to its low water solubility a 1000 mg/L loading rate WSF was used in a limit test. The substance analytics using GC confirmed the low water solubility (< 0.05 mg/L). 10 daphnids were exposed over 96 h. No toxic effects were observed within the range of solubility in water. Thus, the 96h-EL50 value is determined to be > 1000 mg/L based on the nominal and > 0.05 mg/L based on the initially measured test concentration.

Reference 4

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Study period:

1986

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication which meets basic scientific principles. Experimental data for C13 component of Hydrocarbons, C11 -C13 (odd numbered), n-alkanes.

Qualifier:

no guideline followed

Qualifier:

equivalent or similar to guideline

Guideline:

EPA OPPTS 850.1020 (Gammarid Acute Toxicity Test)

Principles of method if other than guideline:

96h acute toxicity test with Chaetogammarus marinus

GLP compliance:

no

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 1000 mg/L loading rate WSF
- Sampling method: 100 mL aliquots were in the same prepared as the test solutions for the test.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution of 1000 mg test substance/L was stirred for 24 hours and the resulting solution was allowed to stand for 4 hrs. The
aqueous solution used for testing was then drained off through a stopcock at the bottom of the vessel. Thus, the WSF of a 1000 mg/L loading was tested. In addition 100 mL aliquots were in the same way run into glass-stoppered 100 mL bottles for test substance analysis.

Test organisms (species):

other aquatic crustacea: Chaetogammarus marinus

Details on test organisms:

TEST ORGANISM
- Source: in-house culture in seaweater
- Age at study initiation (mean and range, SD): young
- Length at study initiation: 5 mm
- Feeding during test
- Food type: Fucus spec.

Test type:

semi-static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

15 °C

pH:

7.5 - 8.3

Dissolved oxygen:

> 6.5 mg/L

Salinity:

2.8%

Nominal and measured concentrations:

Nominal: 1000 mg/L loading rate WAF
Measured (initial): < 0.05 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 mL closed scintillation vials almost completely filled with test solution
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Renewal rate of test solution (frequency/flow rate): Animals surviving after 24h were transferred to fresh test solutions prepared in the same way. This procedure was repeated to add up to a total duration of 96h.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural seawater
- Intervals of water quality measurement: Oxygen and pH were monitored.

EFFECT PARAMETERS MEASURED: Immobility was recorded after 96h test duration.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

EL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

loading rate WSF

Basis for effect:

mobility

Duration:

96 h

Dose descriptor:

EL50

Effect conc.:

> 0.05 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

dissolved

Remarks:

loading rate WAF

Basis for effect:

mobility

Details on results:

No toxic effects were observed within the range of solubility in water.

Validity criteria fulfilled:

not applicable

Conclusions:

The test substance is not toxic to Chaetogammarus marinus within the range of solubility in water.

Executive summary:

The acute toxicity of the test substance was tested in an 96h non-GLP study with Chaetogammarus marinus under semi-static conditions. Due to its low water solubility a 1000 mg/L loading rate WSF was used in a limit test. The substance analytics using GC confirmed the low water solubility (< 0.05 mg/L). 10 daphnids were exposed over 96 h. No toxic effects were observed within the range of solubility in water. Thus, the 96h-EL50 value is determined to be > 1000 mg/L based on the nominal and > 0.05 mg/L based on the initially measured test concentration.

Reference 5

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Study period:

1986

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication which meets basic scientific principles. Experimental data for C13 component of Hydrocarbons, C11 -C13 (odd numbered), n-alkanes.

Qualifier:

no guideline followed

Qualifier:

equivalent or similar to guideline

Guideline:

EPA OPPTS 850.1035 (Mysid Acute Toxicity Test)

Principles of method if other than guideline:

96h acute toxicity test with Mysidopsis bahia

GLP compliance:

no

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 1000 mg/L loading rate WSF
- Sampling method: 100 mL aliquots were in the same prepared as the test solutions for the test.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution of 1000 mg test substance/L was stirred for 24 hours and the resulting solution was allowed to stand for 4 hrs. The
aqueous solution used for testing was then drained off through a stopcock at the bottom of the vessel. Thus, the WSF of a 1000 mg/L loading was tested. In addition 100 mL aliquots were in the same way run into glass-stoppered 100 mL bottles for test substance analysis.

Test organisms (species):

Americamysis bahia (previous name: Mysidopsis bahia)

Details on test organisms:

TEST ORGANISM
- Source: in-house culture in seaweater
- Age at study initiation (mean and range, SD): 4 weeks
- Length at study initiation: 6 mm
- Feeding during test
- Food type: Artemia nauplii

Test type:

semi-static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

20 °C

pH:

7.5 - 8.3

Dissolved oxygen:

> 6.5 mg/L

Salinity:

2.8%

Nominal and measured concentrations:

Nominal: 1000 mg/L loading rate WAF
Measured (initial): < 0.05 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 mL closed scintillation vials almost completely filled with test solution
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Renewal rate of test solution (frequency/flow rate): Animals surviving after 24h were transferred to fresh test solutions prepared in the same way. This procedure was repeated to add up to a total duration of 96h.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural seawater
- Intervals of water quality measurement: Oxygen and pH were monitored.

EFFECT PARAMETERS MEASURED: Immobility was recorded after 96h test duration.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

EL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

loading rate WSF

Basis for effect:

mobility

Duration:

96 h

Dose descriptor:

EL50

Effect conc.:

> 0.05 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

dissolved

Remarks:

loading rate WAF

Basis for effect:

mobility

Details on results:

No toxic effects were observed within the range of solubility in water.

Validity criteria fulfilled:

not applicable

Conclusions:

The test substance is not toxic to Mysidopsis bahia within the range of solubility in water.

Executive summary:

The acute toxicity of the test substance was tested in an 96h non-GLP study with Mysidopsis bahia under semi-static conditions. Due to its low water solubility a 1000 mg/L loading rate WSF was used in a limit test. The substance analytics using GC confirmed the low water solubility (< 0.05 mg/L). 10 daphnids were exposed over 96 h. No toxic effects were observed within the range of solubility in water. Thus, the 96h-EL50 value is determined to be > 1000 mg/L based on the nominal and > 0.05 mg/L based on the initially measured test concentration.

Reference 6

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Study period:

March 1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to a standard guideline without deviations from the protocol and was conducted under GLP guidelines.

Qualifier:

according to guideline

Guideline:

other: ISO (1990) Proposal to TC147/SC5/WG2: Determination of acute lethal toxicicty to marine adult copepods (Arcartia tonsa Dana)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

A water accommodated fraction was prepared for each treatment loading. Test substance was added to test medium and stirred for approximately 20 hours, then allowed to stand for approximately 1 hour before withdrawing an aliquot by siphon from the middle of the aqueous phase to avoid either floating or settled test substance. Seawater used in the study was UV sterilized, filtered to 0.2 um, and obtained from Scapa Flow, Orkney, UK.

Test organisms (species):

other: Arcartia tonsa

Details on test organisms:

Test organisms were 19 days old at test initiation.

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

None

Test temperature:

19.6 to 21.7 degrees C

pH:

8.00 to 8.02

Dissolved oxygen:

97 to 99%

Salinity:

33.7‰ (3.4%)

Nominal and measured concentrations:

Nominal treatment levels included the control and 1000, 3000, 3204, 5596, and 10000 mg/L.

Details on test conditions:

Test vessels were covered 100 ml borosilicate glasss crystallizing dishes containing 50 ml of test medium. 18 to 21 organisms were tested per treatment level and 38 organisms were tested in the control. No more than 11 organisms were added to a test vessel.

Reference substance (positive control):

yes

Remarks:

3,5-dichorophenol

Duration:

24 h

Dose descriptor:

LL50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

LL50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

Mortality results for the definitive test:
Nominal Copepod Total
Loading Mortality Mortality
(mg/L) (@ 24, 48 hrs)* (%)
Control 0, 1 3
1000 2, 3 17
3000 0, 0 0
3204 0, 1 5
5596 1, 1 5
10,000 0, 1 5

*38 organisms tested in the control and 18 to 21 organisms tested in the treatment levels

Results with reference substance (positive control):

The 48-hour LC50 value for 3,5-dichlorophenol was 0.78 mg/L, which compared to the historical laboratory mean concentration of 0.68 mg/L.

Reported statistics and error estimates:

Statistical analyses were not conducted as 50% motality was not reached.

Validity criteria fulfilled:

yes

Conclusions:

Water accommodated fractions (WAFs) of the test substance did not produce 50% mortality with Arcartia tonsa at loading levels ranging between 1000 and 10,000 mg/L after a 48-hour exposure. Therefore, the 48-hour LL50 is reported as >10,000 mg/L.

Executive summary:

Water accommodated fractions (WAFs) of the test substance did not produce 50% mortality with Arcartia tonsa at loading levels ranging between 1000 and 10,000 mg/L after a 48-hour exposure. Therefore, the 48-hour LL50 is reported as >10,000 mg/L. Although the three highest loading levels demonstrated a low degree of mortality, the reported mortality was eqivalent to the control. The 17% mortality reported in the 1000 mg/L loading level was the highest mortality reported in any of the treatment levels, but as this occurred in the lowest level and the treatment levels above it demonstrated either no mortality or mortality that was comparable to the control, the mortality in the lowest loading level is not considered significant.

Description of key information

No toxic effect occurred within the range of solubility in water.

Additional information

No study investigating the short-term toxicity of Hydrocarbons, C11-C13 (odd numbered), n-alkanes, <2% aromatics to aquatic invertebrates is available. Thus, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5 Grouping of substances, a read-across to the two main components undecane (CAS 1120-24-2) and tridecane (CAS 629-50-5) was conducted. The read across is justified since the tested substances are structurally identical to the components of the substance to register. In addition, due to the very high structural similarity of both components (undecane, tridecane) it is unlikely that effects are overlooked by separately testing the components.

In a research project funded by the dutch Ministry of Housing, Physical Planning and Environment the short-term toxicity of n-undecane to Daphnia magna and of n-undecane and n-tridecane to the marine crustacean species Chaetogammarus marinus and Mysidopsis bahia were investigated.

Being a well-established standard test species of the freshwater environment the test results with Daphnia magna (available only for undecane) were used as key information for the hazard assessment of Hydrocarbons, C11 -C13 (odd numbered), n-alkanes, <2% aromatics.

The acute toxicity of n-undecane was tested in an 48h non-GLP study with Daphnia magna under static conditions. Due to its low water solubility a 1000 mg/L loading rate WSF was used in a limit test. The substance analytics using GC confirmed the low water solubility (< 0.05 mg/L). 25 daphnids were exposed over 48 h. No toxic effects were observed within the range of solubility in water. Thus, the 48h-EL50 value is determined to be > 1000 mg/L based on the nominal and > 0.05 mg/L based on the initially measured test concentration.

This result was confirmed by test results of n-undecane and n-tridecane with the marine species Chaetogammarus marinus and Mysidopsis bahia. The short-term toxicity of the test substances were tested in 96h non-GLP studies under semi-static conditions. Due to its low water solubility a 1000 mg/L loading rate WSF was used in a limit test. The substance analytics using GC confirmed the low water solubility of both substances (< 0.05 mg/L). 10 daphnids were exposed over 96h. For both substances and all species tested no toxic effects were observed within the range of solubility in water. Thus, the 96h-EL50 values for all species-substance combinations are determined to be > 1000 mg/L based on the nominal and > 0.05 mg/L based on the initially measured test concentration.

Based on these information it can be concluded that Hydrocarbons, C11-C13 (odd numbered), n-alkanes, <2% aromatics is not considered to be toxic to aquatic invertebrates within its range of solubility in water.

This conclusion is confirmed by additional experimental data showing no acute toxic effects of the structurally similar Hydrocarbons, C11-C14, n-alkanes, <2% aromatics to Acartia tonsa.