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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Description of key information

Key value for chemical safety assessment

EC50 for microorganisms:
430 mg/L
EC10 or NOEC for microorganisms:
38 mg/L

Additional information

A study was performed to assess the effect of the test item on the respiration of activated sewage sludge. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (2010) No. 209 "Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation)".

Following a preliminary range-finding test, activated sewage sludge was exposed to an aqueous solution of the test item at concentrations of 3.2, 10, 32, 100 and 320 mg/L (5 replicates) for a period of 3 hours at temperatures of between 19 and 20 °C with the addition of a synthetic sewage as a respiratory substrate. The rate of respiration was determined after 3 hours contact time and compared to data for the control and a reference item, 3,5-dichlorophenol.

The effect of the test item on the respiration of activated sewage sludge gave a 3-Hour EC10 value of 38 mg/L and a 3-Hour EC50 value of 430 mg/L · The No Observed Effect Concentration (NOEC) after 3 hours exposure was 10 mg/L. The reference item gave a 3-Hour EC50 value of 6.0 mg/L, 95% confidence limits 4.5 - 8.0 mg/L.