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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study conducted in compliance with GLP and OECD Guideline. For justification of read across see section 13.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1995

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Deviations:
no
GLP compliance:
yes
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Reference substance name:
Methyl benzoate
EC Number:
202-259-7
EC Name:
Methyl benzoate
Cas Number:
93-58-3
IUPAC Name:
methyl benzoate

Test animals

Species:
rabbit
Strain:
New Zealand White
Sex:
male/female

Administration / exposure

Type of coverage:
not specified
Vehicle:
unchanged (no vehicle)
Details on dermal exposure:
TEST MATERIAL
- Amount applied: 2000 mg/kg bw

Duration of exposure:
single exposure, test substance was not washed off afterwards
Doses:
one dose, 2000 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
not required
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Animals were weighted on days 1, 2, 4, 7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, organ weights, histopathology

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 2 000 mg/kg bw
Based on:
test mat.
Mortality:
No mortality occurred.
Clinical signs:
No treatment-related findings were recorded.
Body weight:
A decrease in body weights was observed in four male and three female rabbits on day 1 and one female showed a decrease in body weight during days 4 and 7.
Gross pathology:
No abnormal findings were recorded.
Other findings:
- Other observations: Cysts in the oviduct were found in three females which were considered to be normal for this strain of rabbits and therefore not treatment-related.

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
Methyl bezoate was tested for its dermal toxicity in 10 New Zealand White rabbits. It was shown that a single dose of 2000 mg/kg bw did not lead to any deaths, and therefore the LD 50 was found to be >2000 mg/kg bw.
Executive summary:

Methyl bezoate was tested for its dermal toxicity in 10 New Zealand White rabbits (5 males and 5 females). Therefore, a single dose of 2000 mg/kg bw was applied to the rabbits' skin. Loss of test substance was prevented by using a collar. The animals were weighted (on days 1, 2, 4, 7 and 14) and observed during the 14 -day observation period. No deaths occurred. The treated skin sites showed signs of irritaion. A decrease in body weights was observed in four male and three female rabbits on day 1 and one female showed a decrease in body weight during days 4 and 7. At necropsy, no treatment related findings were observed. Therefore, the LD50 after dermal application was found to be greater than 2000 mg/kg bw.