Reaction mass of Sodium 2-(2 or 3-{[(chloroacetyl)amino]methyl}-4-{[4-(cyclohexylamino)-9,10-dioxo-9,10-dihydroanthracen-1-yl]amino}phenoxy)-5-methylbenzenesulfonate and Sodium 2-(3 or 2-{[(chloroacetyl)amino]methyl}-4-{[4-(cyclohexylamino)-9,10-dioxo-9,10-dihydroanthracen-1-yl]amino}phenoxy)-5-methylbenzenesulfonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Specific details on test material used for the study:

None

Analytical monitoring:

no

Details on sampling:

Not available

Vehicle:

not specified

Details on test solutions:

None

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow Trout

Test type:

not specified

Water media type:

not specified

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

No data

Hardness:

No data

Test temperature:

No data

pH:

pH 7-8

Dissolved oxygen:

No data

Salinity:

No data

Nominal and measured concentrations:

0.2, 2, 100 and 200 mg/L

Details on test conditions:

No data

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

other: TL0

Effect conc.:

0.2 mg/L

Nominal / measured:

not specified

Conc. based on:

not specified

Basis for effect:

not specified

Duration:

48 h

Dose descriptor:

other: TL50

Effect conc.:

1.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

other: TL100

Effect conc.:

20 mg/L

Nominal / measured:

not specified

Conc. based on:

not specified

Basis for effect:

not specified

Details on results:

None

Results with reference substance (positive control):

None

Reported statistics and error estimates:

No data

Sublethal observations / clinical signs:

No mortality was seen with the test concentration 0.2 mg/L. While 5 out of 8 fish were found dead when exposed to 2 mg/l. At 20 and 100 mg/L, all the fish exposed were found dead.

Validity criteria fulfilled:

not specified

Conclusions:

The 48 h LC50 was found to be 1.3 mg/L.

Executive summary:

The short term toxicity of FAT 20077/A was evaluated in a study conducted using methodology similar OECD Guideline 203. Three groups of containing 8 fish each were exposed to test concentrations of 0.2, 2, 20 and 100 mg/L for 48 hours. No mortality was seen with the test concentration 0.2 mg/L. While 5 out of 8 fish were found dead when exposed to 2 mg/l. At 20 and 100 mg/L, all the fish exposed were found dead. Based on these findings, the following results were obtained:

TL0 (48 h): 0.2 mg/L

TL50 (48 h): 1.3 mg/L

TL100 (48 h): 20 mg/L

Description of key information

The short term toxicity of FAT 20077/A was evaluated in a study conducted using methodology similar OECD Guideline 203. Three groups of containing 8 fish each were exposed to test concentrations of 0.2, 2, 20 and 100 mg/L for 48 hours. No mortality was seen with the test concentration 0.2 mg/L. While 5 out of 8 fish were found dead when exposed to 2 mg/l. At 20 and 100 mg/L, all the fish exposed were found dead. Based on these findings, the following results were obtained:

TL0 (48 h): 0.2 mg/L

TL50 (48 h): 1.3 mg/L

TL100 (48 h): 20 mg/L

In couple of supporting studies evaluating short term toxicity to fish, the 48 H TL50 obtained were 3.2 and 4.4 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1.3 mg/L