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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
12 - 14 January 2015
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study has been performed according to OECD and EC guidelines and according to GLP principles.
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
Singular samples for possible analysis were taken from all test concentrations (0.1, 1, 10 and 100 mg/L) and the control.

Frequency: at t=0 h and t=48 h
Volume: 2 mL from the approximate centre of the test vessels
Storage: Samples were stored in a freezer until analysis.

At the end of the exposure period, the replicates were pooled at each concentration before sampling.

On the day of analysis, the test samples were defrosted at room temperature. The samples were diluted in a 1:1 (v:v) ratio with acetonitrile and analysed. If necessary, the samples were further diluted with 50/50 (v/v) acetonitrile/water to obtain concentrations within the calibration range.


Vehicle:
no
Details on test solutions:
The test substance was completely soluble in test medium at the concentrations tested.

Preparation of test solutions started with the highest test concentration of 100 mg/L. A 19-minute period of magnetic stirring was applied to ensure complete dissolution. The lower test concentrations were prepared by subsequent dilutions of the highest test concentration in test
medium. The final test solutions were all clear and colourless.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia Magna
- Source: In-house laboratory culture with a known history. At least third generation, obtained by acyclical parthenogenesis under specified breeding conditions.
- Age at study initiation (mean and range, SD): < 24 hours, from parental daphnids of more than two weeks old.

- Method of breeding:
Start of each batch: With newborn daphnids, i.e. less than 3 days old, by placing about 250 of them into 5 litres of medium in an all-glass culture vessel.
Maximum age of the cultures: 4 weeks
Renewal of the cultures: After 7 days of cultivation half of the medium twice a week.
Temperature of medium: 18-22°C
Feeding: Daily, a suspension of fresh water algae.
Medium: M7, as prescribed by Dr. Elendt-Schneider (Elendt, B.-P., 1990: Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus. Protoplasma 154, 25-33).

- Feeding during test: no feeding

ACCLIMATION
- Acclimation period: no
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
None.
Hardness:
180 mg/l expressed as CaCO3
Test temperature:
19- 21 °C
pH:
Start of the test: 7.6 - 7.7
End of the test: 8.1-8.2
Dissolved oxygen:
9.0 - 9.3 mg/L
Salinity:
Not applicable
Nominal and measured concentrations:
The study was carried out as a combined limit/ range-finder test with nominal test concentrations: 0.1, 1, 10 and 100 mg/L.
Since there were no effects observed in all test concentrations, only the highest test concentration was analysed.
Analysis of the samples showed that measured concentrations were stable and in agreement with nominal throughout the test period (102-104%). Results are expressed as nominal concentrations.
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 mL, all-glass
- Aeration: no aeration of the test solutions
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 for the limit test concentrations, 2 for the remaining concentrations
- No. of vessels per control (replicates): 4
- Biomass loading rate: 5 daphnids per vessel containing 80 mL of test solution

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours daily

EFFECT PARAMETERS MEASURED: Immobility at 24 and 48h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Combined limit/range finding study
- Test concentrations: 0.1, 1.0, 10 and 100 mg/L
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: The nominal concentration was confirmed by chemical analysis (recovery 102-104%)
Details on results:
No significant immobilisation was observed during the test. The immobility observed in the control, 0.10, and 100 mg/L did not exceed 10% and were considered biologically insignificant.
Results with reference substance (positive control):
The actual responses in the reference test with K2Cr2O7 (48h-EC50 0.47 mg/L) are within the ranges of the expected responses at the different concentrations. Hence, the sensitivity of this batch of D. magna was in agreement with the historical data collected at WIL Research.
Validity criteria fulfilled:
yes
Remarks:
1. In the control, no daphnids became immobilised. 2. The oxygen concentration at the end of the test was ≥ 3 mg/L in control and test vessels.
Conclusions:
The substance has no intrinsic acute toxic effects up to a nominal test concentration of 100 mg/L.
The nominal 48h-EC50 >100 mg/L.
Executive summary:

The toxicity of the substance to Daphnia magna was investigated under static conditions in a study carried out in accordance with OECD 202 and GLP.

A combined-limit/range finder was carried out with nominal concentrations of 0.1, 1, 10 and 100 mg/L.

The nominal concentration of the highest test concentration was confirmed by chemical analysis (recovery 102 -104%)

No intrinsic acute toxic effects were observed up to and including a nominal test concentration of 100 mg/L.

The nominal 48h-EC50 >100 mg/L.

The study is considered reliable without restrictions.

Description of key information

The toxicity of the substance to Daphnia magna was investigated under static conditions in a study carried out in accordance with OECD 202 and GLP.
The substance has no intrinsic acute toxic effects to Daphnia magna up to a nominal test concentration of 100 mg/L.
The nominal 48h-EC50 >100 mg/L.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

The toxicity of the substance to Daphnia magna was investigated under static conditions in a study carried out in accordance with OECD 202 and GLP.

A combined-limit/range finder was carried out with nominal concentrations of 0.1, 1, 10 and 100 mg/L.

The nominal concentration of the highest test concentration was confirmed by chemical analysis (recovery 102 -104%)

No intrinsic acute toxic effects were observed up to and including a nominal test concentration of 100 mg/L.

The nominal 48h-EC50 >100 mg/L.

The study is considered reliable without restrictions.