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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
October 1987
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP and test guideline compliant study, good documentation, no dose verification performed
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The definitive test concentrations were obtained by transfering appropriate weights of test item directly to the test chambers.
- Controls: blank controls
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): NA
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): NA
- Evidence of undissolved material (e.g. precipitate, surface film, etc): There was was a slight amount of foam present on all test vessels at 0-hour. After 24-hours no foam remained.
- Test solution preparation:
The definitive test concentrations were obtained by transfering appropriate weights of test item directly to the test chambers. Fish were randomly added to the test solutuions within 30 minutes after test solution preparation. All test concentrations were based on the total compound, i.e., not corrected for purity or undissolved material.
Test organisms (species):
Lepomis macrochirus
Details on test organisms:
TEST ORGANISM
- Common name: bluegill sunfish
- Source: Osage Catfisheries, Osage Beach
- Length at study initiation (length definition, mean, range and SD): 21 (+/- 1.6 ) mm
- Weight at study initiation (mean and range, SD): 0.21 (+/- 0.057) g/fish
- Feeding during test: the fish were not fed durung the exposure



ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: standrad commercial fish food
- Feeding frequency: daily, discontinued 48 to 96 hours prior to testing


QUARANTINE (wild caught)
- Duration: at least 14 days
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
40-48 mg/L as CaCO3
Test temperature:
21 - 22°C
pH:
7.2 - 7.7
Dissolved oxygen:
5.9 - 8.6 mg/L
Nominal and measured concentrations:
nominal concentrations: 0 (control), 18, 24, 32, 42, and 56 mg CT-317-87/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 5 gallon glass vessels containing 15 L test solution
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): NA
- Biomass loading rate: 0.14 g/L


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: soft reconstituted water
Following amounts were added to deionized water:
NaHCO3: 48 mg/L
CaSO4*2H2O: 30 mg/L
MgSO4: 30 mg/L
KCl: 2 mg/L
- Metals: measured, levels below levels of concern
- Pesticides:measured, levels below levels of concern
- Alkalinity: 25 - 35 mg/L as CaCO3
- Intervals of water quality measurement: measured at 24, 48 and 96 hours


OTHER TEST CONDITIONS
- Adjustment of pH: no



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
mortality and behavioral changes

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.3
- Justification for using less concentrations than requested by guideline: NA
- Range finding study: two range finding studies were performed:
- Test concentrations:
first range finder: 10, 100, 1000 mg CT-317-87/L
second range finder: 32 mg CT-317-87/L
- Results used to determine the conditions for the definitive study:
first range finder: 10, 100, 1000 mg CT-317-87/L with 0/5, 5/5, 5/5 dead fish after 24 hours to 96 hours;
second range finder: 32mg CT-317-87/L with 3/5 dead fish after 96 hours
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
18 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
36 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% C.I.: 32 - 42 mg test material/L
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
15.5 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
31 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% C.I.: 25 - 38 mg test material/L
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
14.5 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
29 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% C.I.: 25 - 36 mg test material/L
Details on results:
- Behavioural abnormalities: none reported
- Observations on body length and weight: NA
- Other biological observations: behavioral changes (surfacing, exitability) at all test concentrations including the lowest test concetration of 18 mg CT-317-87/L
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: behavioral changes (surfacing, exitability) were observed at all test concentrations including the lowest test concetration of 18 mg CT-317-87/L
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: There was was a slight amount of foam present on all test vessels at 0-hour. After 24-hours no foam remained.
- Effect concentrations exceeding solubility of substance in test medium: No information provided.
Reported statistics and error estimates:
The LC50 values for CT-316-87 were determined using the probit method.

Table 1 Nominal concentration of CT-317-87 and corresponding concentrations of CH-SSS in the test solutions (calculated for 97% purity)

 

Nominal concentration

[mg CT-317-87/L]

Nominal concentration

[mg CH-SSS/L]

0 (Control)

0 (Control)

18

9

24

12

32

16

42

21

56

28

 

 

Table 2   Temperature, dissolved oxygen and pH values measured in the test vessels at different time intervals

 

Nominal concentration

[mg CT-317-87 /L]

0 hours

48 hours

96 hours

Temp

[°C]

DO

[mg/L]

pH

Temp

[°C]

DO

[mg/L]

pH

Temp

[°C]

DO

[mg/L]

pH

0 (Control)

21

8.6

7.6

22

7.2

7.3

22

6.0

7.2

18

21

8.6

7.7

22

7.1

7.3

22

5.9

7.2

24

-

-

-

22

7.1

7.2

22

5.9

7.2

32  21  8.6  7.6  22 7.3 7.3  22 6.1 7.2
42  -  -  - -  - -  -  -  -
 56  21  8.6 7,7  -

 

 

Table 3 Percent mortality at the individual observation intervals

 

Nominal concentration

[mg CT-317-87 /L]

Cumulative Percent Mortality

24 hours

48 hours

96 hours

0 (Control)

0

0

0

18

10

20

30

24

0

0

10

32

10

40

50

42

100

100

100

56

100

100

100

 

Validity criteria fulfilled:
yes
Remarks:
the control validity criteria were fulfilled, the DO concentration criteria not at higher concentrations
Conclusions:
96-hour LC50 (Lepomis macrochirus)= 14.5 mg/L
Executive summary:

The acute toxicity of CT-317 -87 - which contains 50% CAS 37294 -49 -8 - to bluegill sunfish, Lepomis macrochirus, was determined in a static test. The test was performed according to OECD 203. No deviations to the guideline were reported. The test solutions were not aerated. No dose-verification analysis was performed. The test was performed with a control and nominal 18, 24, 32, 42, and 56 mg CT-317 -87/L corresponding to 9, 12, 16, 21, and 28 mg CAS 37294 -49 -8/L. During the study, the temperature of the test solutions ranged from 21 to 22°C. During the study the oxygen concentration ranged from 5.9 to 8.6 mg/L. After 96 hours of exposure, 0, 3, 1, 5, 10 and 10 out of 10 fish had died in the control, and 18, 24, 32, 42, and 56 mg CT-317 -87/L treatment groups. The 96 h LC50 was determined to be 29 mg CT-317 -87/L corresponding to 14.5 mg CAS 37294 -49 -8/L. The concentrations were based on nominal concentrations.

The results are considered to be reliable with restrictions for the risk assessment.

Description of key information

short-term toxicity to fish LC50: 14.5 mg/L

Key value for chemical safety assessment

LC50 for freshwater fish:
14.5 mg/L

Additional information

The acute toxicity of CT-317 -87 - which contains 50% CAS 37294 -49 -8 - to bluegill sunfish, Lepomis macrochirus, was determined in a static test. The test was performed according to OECD 203. No deviations to the guideline were reported. The test solutions were not aerated. No dose-verification analysis was performed. The test was performed with a control and nominal 18, 24, 32, 42, and 56 mg CT-317 -87/L corresponding to 9, 12, 16, 21, and 28 mg CAS 37294 -49 -8/L. During the study, the temperature of the test solutions ranged from 21 to 22°C. During the study the oxygen concentration ranged from 5.9 to 8.6 mg/L. After 96 hours of exposure, 0, 3, 1, 5, 10 and 10 out of 10 fish had died in the control, and 18, 24, 32, 42, and 56 mg CT-317 -87/L treatment groups. The 96 h LC50 was determined to be 29 mg CT-317 -87/L corresponding to 14.5 mg CAS 37294 -49 -8/L. The concentrations were based on nominal concentrations.

The results are considered to be reliable with restrictions for the risk assessment.