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EC number: 241-442-6 | CAS number: 17418-58-5
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: screening test, other
- Remarks:
- BOD5/COD
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- September 21st, 1977
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Remarks:
- Result considered plausible for a risk assessment.
- Qualifier:
- according to guideline
- Guideline:
- other: internal method A-15
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- other: internal method A-16
- Deviations:
- not specified
- Principles of method if other than guideline:
- The test has been conducted according to the internal method A-15, Biological Oxygen demand (BOD5), similar to the OECD guidelines.
The determination of the biological oxygen demand was performed according to published methods, which were adapted and standardized for the specific needs of the company, well before Test Guidelines for these methods were established by the OECD beginning 1981.
The results of these studies were reported until the early 1980s in summary reports which do not present details of the methods used, but focus on a summary presentation of results.
This statement is intended to describe the methods used and give some guidance on the interpretation of the results described in the above mentioned summary reports. - GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: bacteria from the underrun of a sewage treatment plant
- Details on inoculum:
- The underrun is filtrated through a Whatman GF/A filter before incubation and 2 ml of the filtrate should be used for inoculation of 1 liter of dilution solution (105 bacteria/ml). The resulting dilution water must be aerated for 2 to 3 days until it is totally ablated and saturated with oxygen (about 8 mg/l oxygen).
To avoid algal growth the dilution water have been kept in the dark. - Duration of test (contact time):
- ca. 5 d
- Initial conc.:
- ca. 500 mg/L
- Based on:
- other: BOD5
- Initial conc.:
- ca. 5 mg/L
- Based on:
- COD
- Initial conc.:
- ca. 51 mg/L
- Based on:
- other: TOC
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Solution 1:
8.5 g KH2PO4, 21.75 g K2HPO4 33.4 g Na2HPO4x2H2O and 1.7 g NH4Cl are dissolved in 500 ml deionized water and filled up to a total volume of 1 liter. The pH-value should be 7.2
Solution 2:
22.5 g MgSO4x7H2O is dissolved in deionized water and adjusted to a total volume of 1 liter.
Solution 3:
27.5 g CaCl2 is dissolved in deionized water and adjusted to a total volume of 1 liter.
Solution 4:
0.25 g FeCl3 x6H2O is dissolved in deionized water and adjusted to a total volume of 1 liter.
- Preparation:
1 ml of solutions 1-4 are added to 1 liter of deionized water and inoculated with bacteria from the underrun of a sewage treatment plant. The underrun is filtrated through a Whatman GF/A filter before incubation and 2 ml of the filtrate should be used for inoculation of 1 liter of dilution solution (105 bacteria/ml). The resulting dilution water must be aerated for 2 to 3 days until it is totally ablated and saturated with oxygen (about 8 mg/L oxygen). To avoid algal growth the dilution water have beene kept in the dark.
- pH adjusted: yes to 7 - Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 10.4
- Sampling time:
- 5 d
- Parameter:
- BOD5
- Value:
- ca. 10.4 mg O2/g test mat.
- Parameter:
- COD
- Value:
- ca. 1 397 mg O2/g test mat.
- Parameter:
- BOD5*100/COD
- Value:
- ca. 1 mg O2/g test mat.
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- BOD5 = 10.4 mg/g O2
COD = 1397 mg/g O2
BOD5 / COD = ca. 0.01 - Executive summary:
The substance was screened for ready biodegradation potential in the BOD5 and COD test.
The following results were obtained: BOD5 = 10.4 mg/g O2, COD = 1397 mg/g O2, BOD5 / COD = 0.01.
Therefore, the test substance shows no evidence of readily biodegradation.
- Endpoint:
- biodegradation in water: screening test, other
- Remarks:
- BOD5/COD
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- April 24th, 1978
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Remarks:
- Result considered plausible for a risk assessment.
- Qualifier:
- according to guideline
- Guideline:
- other: internal method A-15
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- other: internal method A-16
- Principles of method if other than guideline:
- The test has been conducted according to the internal method A-15, Biological Oxygen demand (BOD5), similar to the OECD Guidelines
The determination of the biological oxygen demand was performed according to published methods, which were adapted and standardized for the specific needs of the company, well before Test Guidelines for these methods were established by the OECD beginning 1981.
The results of these studies were reported until the early 1980s in summary reports which do not present details of the methods used, but focus on a summary presentation of results.
This statement is intended to describe the methods used and give some guidance on the interpretation of the results described in the above mentioned summary reports. - GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: bacteria from the underrun of a sewage treatment plant
- Details on inoculum:
- The underrun is filtrated through a Whatman GF/A filter before incubation and 2 ml of the filtrate should be used for inoculation of 1 liter of dilution solution (105 bacteria/ml). The resulting dilution water must be aerated for 2 to 3 days until it is totally ablated and saturated with oxygen (about 8 mg/l oxygen).
To avoid algal growth the dilution water have been kept in the dark. - Duration of test (contact time):
- ca. 5 d
- Initial conc.:
- ca. 200 mg/L
- Based on:
- other: BOD5
- Initial conc.:
- ca. 5 mg/L
- Based on:
- COD
- Initial conc.:
- ca. 56.5 mg/L
- Based on:
- other: TOC
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Solution 1:
8.5 g KH2PO4, 21.75 g K2HPO4 33.4 g Na2HPO4x2H2O and 1.7 g NH4Cl are dissolved in 500 ml deionized water and filled up to a total volume of 1 liter. The pH-value should be 7.2
Solution 2:
22.5 g MgSO4x7H2O is dissolved in deionized water and adjusted to a total volume of 1 liter.
Solution 3:
27.5 g CaCl2 is dissolved in deionized water and adjusted to a total volume of 1 liter.
Solution 4:
0.25 g FeCl3 x6H2O is dissolved in deionized water and adjusted to a total volume of 1 liter.
- Preparation:
1 ml of solutions 1-4 are added to 1 liter of deionized water and inoculated with bacteria from the underrun of a sewage treatment plant. The underrun is filtrated through a Whatman GF/A filter before incubation and 2 ml of the filtrate should be used for inoculation of 1 liter of dilution solution (105 bacteria/ml). The resulting dilution water must be aerated for 2 to 3 days until it is totally ablated and saturated with oxygen (about 8 mg/L oxygen). To avoid algal growth the dilution water have beene kept in the dark.
- pH adjusted: yes to 7 - Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 20
- Sampling time:
- 5 d
- Parameter:
- BOD5
- Value:
- ca. 20 mg O2/g test mat.
- Parameter:
- COD
- Value:
- ca. 1 401 mg O2/g test mat.
- Parameter:
- BOD5*100/COD
- Value:
- ca. 1 mg O2/g test mat.
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- BOD5 = 20 mg/g O2
COD = 1401 mg/g O2
BOD5 / COD = ca. 0.01 - Executive summary:
The substance was screened for ready biodegradation potential in the BOD5 and COD test.
The following results were obtained: BOD5 = 20 mg/g O2, COD = 1401 mg/g O2, BOD5 / COD = 0.01.
Therefore, the test substance shows no evidence of ready biodegradation.
- Endpoint:
- biodegradation in water: screening test, other
- Remarks:
- BOD5/COD
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- July 30th, 1980
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Remarks:
- Result considered plausible for a risk assessment.
- Qualifier:
- according to guideline
- Guideline:
- other: internal method A-16
- Deviations:
- not specified
- Principles of method if other than guideline:
- The test has been conducted according to the internal method A-15, Biological Oxygen demand (BOD5), similar to the OECD Guidelines
The determination of the biological oxygen demand was performed according to published methods, which were adapted and standardized for the specific needs of the company, well before Test Guidelines for these methods were established by the OECD beginning 1981.
The results of these studies were reported until the early 1980s in summary reports which do not present details of the methods used, but focus on a summary presentation of results.
This statement is intended to describe the methods used and give some guidance on the interpretation of the results described in the above mentioned summary reports. - GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: bacteria from the underrun of a sewage treatment plant
- Details on inoculum:
- The underrun is filtrated through a Whatman GF/A filter before incubation and 2 ml of the filtrate should be used for inoculation of 1 liter of dilution solution (105 bacteria/ml). The resulting dilution water must be aerated for 2 to 3 days until it is totally ablated and saturated with oxygen (about 8 mg/l oxygen).
To avoid algal growth the dilution water have been kept in the dark. - Duration of test (contact time):
- ca. 5 d
- Initial conc.:
- ca. 200 mg/L
- Based on:
- other: BOD5
- Initial conc.:
- ca. 5 mg/L
- Based on:
- COD
- Initial conc.:
- ca. 54.5 mg/L
- Based on:
- other: TOC
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Solution 1:
8.5 g KH2PO4, 21.75 g K2HPO4 33.4 g Na2HPO4x2H2O and 1.7 g NH4Cl are dissolved in 500 ml deionized water and filled up to a total volume of 1 liter. The pH-value should be 7.2
Solution 2:
22.5 g MgSO4x7H2O is dissolved in deionized water and adjusted to a total volume of 1 liter.
Solution 3:
27.5 g CaCl2 is dissolved in deionized water and adjusted to a total volume of 1 liter.
Solution 4:
0.25 g FeCl3 x6H2O is dissolved in deionized water and adjusted to a total volume of 1 liter.
- Preparation:
1 ml of solutions 1-4 are added to 1 liter of deionized water and inoculated with bacteria from the underrun of a sewage treatment plant. The underrun is filtrated through a Whatman GF/A filter before incubation and 2 ml of the filtrate should be used for inoculation of 1 liter of dilution solution (105 bacteria/ml). The resulting dilution water must be aerated for 2 to 3 days until it is totally ablated and saturated with oxygen (about 8 mg/L oxygen). To avoid algal growth the dilution water have beene kept in the dark.
- pH adjusted: yes to 7 - Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 19
- Sampling time:
- 5 d
- Parameter:
- BOD5
- Value:
- ca. 19 mg O2/g test mat.
- Parameter:
- COD
- Value:
- ca. 1 491 mg O2/g test mat.
- Parameter:
- BOD5*100/COD
- Value:
- ca. 1 mg O2/g test mat.
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- BOD5 = 19 mg/g O2, COD = 1491 mg/g O2, BOD5 / COD = approx. 0.01
- Executive summary:
The substance was screened for ready biodegradation potential in the BOD5 and COD test.
The following results were obtained: BOD5 = 19 mg/g O2, COD = 1491 mg/g O2, BOD5 / COD = approx. 0.01.
Therefore, the test substance shows no evidence of ready biodegradation.
Referenceopen allclose all
Description of key information
Not readily biodegradable
Key value for chemical safety assessment
Additional information
The substance was screened for ready biodegradation potential in three BOD5 and COD tests (1977, 1978 and 1980). All tests gave BOD5 / COD ratios of approximately 0.01.
According to the CLP Regulation EC n. 1272/2008, Annex I, Part 4, subsection 4.1.2.9.2. which states, "One way of demonstrating rapid degradation utilises the biodegradation screening tests designed to determine whether an organic substance is ‘readily biodegradable’. Where such data are not available, a BOD(5 days)/COD ratio ≥ 0,5 is considered as indicative of rapid degradation."
The ratios BOD5/COD are approximately 0.01, this value is less than 0.5 as given in the above regulation. Therefore, the test substance shows no evidence of rapid biodegradation.
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