4-allylveratrole

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

2 rats per sex and dose / poorly documented

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

4-allylveratrole

EC Number:

202-223-0

EC Name:

4-allylveratrole

Cas Number:

93-15-2

Molecular formula:

C11H14O2

IUPAC Name:

1,2-dimethoxy-4-(prop-2-en-1-yl)benzene

Test material form:

liquid

Specific details on test material used for the study:

Purity of each material was checked in the Organic Chemicals Synthesis Laboratory, AEQI, in Beltsville by gas chromatography: > 98%

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 150-240 g
- Fasting period before study: 16 hr before dosing.
- Housing: Individually in suspended wire-mesh cages.

Administration / exposure

Route of administration:

other: Directly into the stomachs with a hypodermic syringe that had a ball-tipped intubation needle.

Vehicle:

unchanged (no vehicle)

Doses:

From 600 to 3038 mg/kg bw

No. of animals per sex per dose:

2 rats per sex and dose.

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

1 179 mg/kg bw

Based on:

test mat.

95% CL:

927.9 - 1 430.1

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

The oral LD50 was determined to be 1179 mg/kg bw in rats.

Executive summary:

An acute oral toxicity test was performed in Sprague-Dawley rats. Test item up to 3038 mg/kg bw was administered to two rats per sex and per dose, directly into the stomachs with a hypodermic syringe that had a ball-tipped intubation needle. The animals were observed for 14 days after exposure. The oral LD50 was determined to be 1179 (± 251.1) mg/kg bw in rats.