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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Key value taken from study data performed using the closed bottle test in accordance with OECD Guideline 301D, EU Method C.4-E and US EPA procedure OPPTS 835.3110.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

Under the test conditions the percentage biodegradation of CUCURBIT[8]URIL reached a mean of 5.9 % after 28 days based on its ThODNO3.

Therefore the test item is considered not readily biodegradable. According to the test guidelines the pass level for ready biodegradability is removal of 60 % ThOD.

The reference item Sodium benzoate was sufficiently degraded to a mean of 73.3 % after 14 days, and to a mean of 86.7 % after 28 days of incubation, based on ThODNH4, thus confirming the suitability of the used activated sludge inoculum.

In the toxicity control containing both, the test item and the reference item Sodium benzoate, a mean of 32.7 % biodegradation was noted within 14 days and 42.1 % biodegradation after 28 days of incubation.

According to the test guidelines the test item can be assumed to be not inhibitory at the applied concentration level of 2.7 mg/L on the activated sludge microorganisms because degradation was >25 % within 14 days.