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Short-term toxicity to fish

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Description of key information

No toxic effects up to the limit of water solubility (< 0.05 mg/L in aqua dest) for Danio rerio, read across

Key value for chemical safety assessment

Additional information

As no study for assessing the acute toxicity to fish of 2-Butenedioic acid (2E)-, di-C12-14-alkyl esters (List No 938-575-3) is available, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5 Grouping of substances, a read-across to the structurally related category members 2-Butenedioic acid (E)-, didodecyl ester (CAS 2402-58-6, MW 453 g/mol), 2-Butenedioic acid (E)-, ditetradecyl ester (CAS 10341-03-4, MW 509 g/mol) and 2-Butenedioic acid (E)-, di-C18-22-alkyl esters (CAS 68921-53-9, MW 621-733 g/mol) was conducted. 2-Butenedioic acid (2E)-, di-C12-14-alkyl esters (List No 938-575-3) exhibits a molecular weight range of 452.72 - 508.82 g/mol. Thus, the read across is covered by interpolation for this substance. The read across is justified due to a) the structural similarity of the category members and b) similar physico-chemical properties resulting in a similar environmental fate and ecotoxicity profile.

Further justification is given in the endpoint summary 6.1 and within the category justification in section 13.

The study with 2-Butenedioic acid (E)-, didodecyl ester (CAS 2402-58-6) was conducted according to GLP and EU method C.1 under semi-static conditions (Scholz, 1995). A 96 h-LC50 value of 2200 mg/L was determined for Danio rerio on the basis of nominal test concentrations.

A study with 2-Butenedioic acid (E)-, ditetradecyl ester (CAS 10341-03-4) was conducted according to GLP and OECD 203 (limit test) using Danio rerio as test organism (Wiech, 2012).No analytical determinations were performed since the solubility of the test item is below the limit of quantification (0.04 mg/L). No sub-lethal or lethal effects were observed in the control and at 100 mg/L. Thus the 96 h-LC50 is assessed as > 100 mg/L.

The study with 2-Butenedioic acid (E)-, di-C18-22-alkyl esters (CAS 68921-53-9) was performed under semi-static conditions according to GLP and EU method C.1 using Brachydanio rerio as test organism as well (Scholz, 1995).Concentrations of 1000, 3000 and 10000 mg/L test material were directly added to the test vessels. No mortality was observed in any treatment and the control during the test duration of 96 hours. Accordingly, the 96 h-LC50 is determined to be > 10000 mg/L.

The results of all three studies show distinctly, that no effects occur in the range of water solubility and well above the water solubility.

Due to the structural and physico-chemical property similarities with the tested category members 2-Butenedioic acid (E)-, didodecyl ester (CAS 2402-58-6), 2-Butenedioic acid (E)-, ditetradecyl ester (CAS 10341-03-4) and 2-Butenedioic acid (E)-, di-C18-22-alkyl esters (CAS 68921-53-9), this result is also applicable to read across to 2-Butenedioic acid (2E)-, di-C12-14-alkyl esters (List No 938-575-3).