3-chloropropionyl chloride

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP, similar to guideline study, available as unpublished report, minor restrictions in design and/or reporting but otherwise adequate for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

Rats (5/sex/dose) were exposed to a single oral dose (six different dose levels) of the test substance. The rats were then observed for 14 days. Mortality, body weight, and clinical signs were recorded. In addition necropsy was performed on all animals.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-chloropropionyl chloride

EC Number:

210-890-4

EC Name:

3-chloropropionyl chloride

Cas Number:

625-36-5

Molecular formula:

C3H4Cl2O

IUPAC Name:

3-chloropropanoyl chloride

Details on test material:

- Name of test material (as cited in study report): 3-Chlorpropionsäurechlorid

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Hagemann + Wiga
- Weight at study initiation: means of the dose groups: males: 170-250 g; females: 180-190 g
- Diet: Herilan MRH-Haltung; H.Eggersmann KG
- Fasting period before study: 15-20 hours

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

olive oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 2, 9.28, 13.62, 20, 29.4, and 43 % w/v
- Amount of vehicle (if gavage): 5 mL/kg

MAXIMUM DOSE VOLUME APPLIED: 5 mL/kg

Doses:

100, 464, 681, 1000, 1470, 2150 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: weighing was performed on day 0, day 2-4, day 7 and day 13
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 1 000 - < 1 470 mg/kg bw

Based on:

test mat.

Mortality:

- No deaths occurred in animals dosed with 100, 464, 681 and 1000 mg/kg bw
- 1470 mg/kg bw: 5/5 male and 3/5 female animals died
- 2150 mg/kg bw: all animals died

Clinical signs:

other: - Animals dosed with 100 and 464 mg/kg bw were free of clinical signs - At 681 mg/kg bw reddened skin and roughed fur was observed - At 1000 mg/kg bw dyspnoea, apathy, staggering, reddened skin, roughed fur and poor general condition was observed - At 147

Gross pathology:

animals that died:
- heart: acute dilation right, acute congestive hyperaemia
- lung: acute exhalation median grade
- stomach: extensive haemorrhagic gastritis due to corrosion
- intestine: partially necrotic and partially reddened mucous membrane, content bloody

sacrificed animals:
- 100 - 681 mg/kg bw: without findings
- 1000 and 1470 mg/kg bw: forefront of the gastroesophageal vestibule thickened, isolated cases of gastroesophageal vestibule adhered to liver, spleen and peritoneum