2(5H)-Furanone, 5-hydroxy-4-methyl-

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

5-hydroxy-4-methyl-2,5-dihydrofuran-2-one

EC Number:

609-869-6

Cas Number:

40834-42-2

Molecular formula:

C5 H6 O3

IUPAC Name:

5-hydroxy-4-methyl-2,5-dihydrofuran-2-one

Details on test material:

- Name of test material (as cited in study report): Hydroxybutenolid
- Molecular formula (if other than submission substance): C5H6O3
- Physical state: solid
- Analytical purity: 92.7 %
- Purity test date:
- Porduct No.: 068039
- Expiration date of the lot/batch: no limit

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Preparation of the inoculum :
The inoculum was washed, brought to a concentration of 5 g/I dry substance and aerated during the night. 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/I dry substance in the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test conditions

Test temperature:

20 +/- 2 °C

pH:

6.5 - 7.7

Nominal and measured concentrations:

Nominal: 0, 9.6, 100, 248, 496, 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer-vessel
- Type: closed
- Material, size, headspace, fill volume: 250 ml
- Aeration: yes
- No. of organisms per vessel:
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3
- Biomass loading rate: 1 g/Ldry substance

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 209

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen consumption

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

ca. 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

ca. 800 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

30 min

Dose descriptor:

EC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate