Registration Dossier
Registration Dossier
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Reaction mass of tetrasodium 7,7'-(carbonyldiimino)bis[4-hydroxy-3-[(2-methyl-4-sulphonatophenyl)azo]naphthalene-2-sulphonate] and tetrasodium 4-[[1-hydroxy-6-[[[[5-hydroxy-6-[(2-methyl-4-sulphonatophenyl)azo]-7-sulphonato-2-naphthyl]amino]carbonyl]amino]-3-sulphonato-2-naphthyl]azo]benzoate and tetrasodium 4,4'-[carbonylbis[imino(1-hydroxy-3-sulphonatonaphthalene-6,2-diyl)azo]]dibenzoate
EC number: 942-930-8 | CAS number: -
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The 48-h EC50 value is >108 mg/L (nominal) in aquatic invertebrates after correction for the percentage active ingredient in the tested substance.
Key value for chemical safety assessment
Additional information
The acute toxicity to aquatic invertebrates was determined in a limit test according to OECD guideline No 202 and in compliance with GLP criteria (BASF, 2015). In this study daphnids (D. magna, 40 per concentration) were exposed to nominal concentrations of 0 (control) and 120 mg/L for 48 hours under static conditions. Analytical confirmation of nominal test concentrations showed that all test concentrations remained well within ±20% of nominal concentrations throughout the test. Therefore, effect concentrations are expressed as nominal. Immobilization was recorded after 24 and 48 hours exposure. No signs of immobilization were observed throughout the test in the test group and controls. Based on these findings the 48 -h EC50 value was determined at >120 mg/L. Based on a reported purity of the substance of ca. 90%, the 48 -h EC50 value is >108 mg/L (nominal).
In another limit test according to the same study design, daphnids were exposed to nominal test concentrations of 0 (control) and 100 mg/L. In this study also no immobilization and/or abnormal behaviour of Daphnia was observed throughout the test. However, the test substance contained only 15.8% active ingredient and the measured test concentration was determined at 10.7 mg/L which is well below the upper cut-off value for environmental classification. Therefore it was decided to redo the test (of which the results are presented above).
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