reaction mass of 3-methyl-4-(2,6,6-trimethylcyclohex-2-en-1-yl)but-3-en-2-one and 1-(2,6,6-trimethylcyclohex-2-en-1-yl)pent-1-en-3-one

Dermal absorption

Administrative data

Endpoint:

dermal absorption in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: no guideline study

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

1984

Reference 2

Reference Type:

publication

Title:

Fragrance material review on methyl ionone (mixture of isomers)

Author:

J. Lalko, A. Lapczynski , D. McGinty, S. Bhatia, C.S. Letizia, A.M. Api

Year:

2007

Bibliographic source:

Food and Chemical Toxicology 45: S300–S307

Materials and methods

Test material

Test material information

1

Reference substance name:

(1E)-1-(2,6,6-trimethylcyclohex-2-en-1-yl)pent-1-en-3-one; (3E)-3-methyl-4-(2,6,6-trimethylcyclohex-2-en-1-yl)but-3-en-2-one

EC Number:

942-741-0

Cas Number:

1335-46-2

Molecular formula:

C14H22O

IUPAC Name:

(1E)-1-(2,6,6-trimethylcyclohex-2-en-1-yl)pent-1-en-3-one; (3E)-3-methyl-4-(2,6,6-trimethylcyclohex-2-en-1-yl)but-3-en-2-one

Specific details on test material used for the study:

Test material was a mixture of 14C labeled and non-radioactive material dissolved in ethanol at a concentration of 10%.

Radiolabelling:

yes

Remarks:

C14

Test animals

Species:

rat

Strain:

other: naked

Administration / exposure

Vehicle:

ethanol

Duration of exposure:

1, 6 or 16 hours

Doses:

10% in ethanol

Results and discussion

Any other information on results incl. tables

600 µg/cm2: skin absorption

After a 1 hour contact with naked rat skin approximately 74% of the test material applied was recovered with 308.76 ug/cm2 found on the skin surface, 27.11 ug/cm2 found in the horny layer of the skin, 107.13 ug/cm2 found in the remaining skin tissue layers, and 0.04 ug/cm2 found in the chamber liquid.

After 6 hours contact with naked rat skin approximately 74% of the test material applied was recovered with 195.17 ug/cm2 found on the skin surface, 21.56 ug/cm2 found in the horny layer of the skin, 219.94 ug/cm2 found in the remaining skin tissue layers, and 3.96 ug/cm2 found in the chamber liquid.

After 16 hours contact with naked rat skin approximately 60% of the test material applied was recovered with 70.35 ug/cm2 found on the skin surface, 32.27 ug/cm2 found in the horny layer of the skin, 249.75 ug/cm2 found in the remaining skin tissue layers, and 7.48 ug/cm2 found in the chamber liquid.

The unrecovered test material was presumably lost by evaporation from the skin surface.

	recovery [%]	skin surface   [µg/cm²]	horny layer   [µg/cm²]	remaining skin tissue layers [µg/cm²]	chamber liquid   [µg/cm²]
1 hour	74	308.76	27.11	107.13	0.04
6 hours	74	195.17	21.56	219.94	3.96
16 hours	60	70.35	32.27	249.75	7.48