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EC number: 200-574-4 | CAS number: 64-04-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 15 June 2012 to 18 june 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- DL-α-methylbenzylamine
- EC Number:
- 210-545-8
- EC Name:
- DL-α-methylbenzylamine
- Cas Number:
- 618-36-0
- Molecular formula:
- C8H11N
- IUPAC Name:
- 1-phenylethanamine
- Details on test material:
- - Name of test material (as cited in study report): 1-Phenylethylamine
- Physical state: liquid, colorless to yellow
- Analytical purity: >=99%
- Isomers composition: R:S form = 0.345 : 99.655 area%.
- Lot/batch No.: O 2932 (production date: 20 Oct 2011)
- Expiration date of the lot/batch: 20 Oct 2013
- Stability under test conditions: test item was stable under test conditions as proven by chemical analysis
- Storage condition of test material: room temperature, dry, protected from light
- Other: solubility in water: 54.4 g/L (25 °C)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all concentrations at test initiation and test termination
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution (1 mg/mL); test item dissolved in test medium (AAP); solution (0.1 mg/mL) prepared from stock solution by dilution with test medium; both solutons used to prepare test solutions in glass flasks of 500 mL total volume
- Controls: negative control
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: SAG 61.81
- Source (laboratory, culture collection): laboratory culture: standard laboratory culture of the Institute of Industrial Organic Chemistry, Branch Pszczyna, Department of Ecotoxicology, Laboratory of Aquatic Toxicology; originally supplied by Algae Collection University of Göttingen, Germany
- Age of inoculum (at test initiation): 4 d
- Method of cultivation: cultured in AAP medium at 21 to 24 °C under sterile conditions, renewal twice a week
ACCLIMATION
- Culturing media and conditions (same as test or not): yes
- Any deformed or abnormal cells observed: no data
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22.2 to 22.7 ºC
- pH:
- pH of control: 7.22 to 9.09
- Nominal and measured concentrations:
- nominal: 0 (control), 0.2, 0.6, 1.8, 5.6, 16.7 and 50.0 mg/L
measured: test initiation: 90.0 to 105.6% of nominal concentration ; test termination: 91.7 to 99.8% of nominal concentration - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Type (delete if not applicable): open (air permeable stoppers )
- Material, size, headspace, fill volume: glass, capacity: 250 mL; fill volume: 100 mL
- Aeration: no; but vessels were shaken continuously (70 rpm)
- Initial cells density: 1 x 10^4cells/mL
- Control end cells density:
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 7
GROWTH MEDIUM
- Standard medium used: yes (AAP)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AAP medium
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: continuous illumination
- Light intensity and quality: 5500 to 6960 lx, fluorescent light source of six 24 W light bulbs
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: algae cell counting in a Bűrker chamber by microscopic observation at 24, 48 and 72 hours of exposure
- Other: algal biomass: absorbance at wavelength (lambda) of 670 nm
TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 3
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study
- Test concentrations: first test: 0.1, 1.0, 10 and 100 mg/L; second test: 0.26, 0.64, 1.60, 4.0 and 10.0 mg/L; third test: 0.26, 0.64, 1.60, 4.0, 10.0 and 25.0 mg/L
- Results used to determine the conditions for the definitive study: inhibition for yield (calculated from average algae cell number) compared with the control: first test: -3.8% in 0.1 mg/L, 24.2% in 1.0 mg/L, 99.0% in 10.0 mg/L and 99.8% in 100.0 mg/L; second test: 4.8% in 0.26 mg/L, 5.7% in 0.64 mg/L, 27.3% in 1.6 mg/L, 21.9% in 4.0 mg/L and 51.0% in 10.0 mg/L; third test: 3.1% in 0.26 mg/L, 19.1% in 0.64 mg/L, 22.1% in 1.6 mg/L, 31.0% in 4.0 mg/L, 52.8% in 10.0 mg/L and 92.7% in 25.0 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 31.66 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 28.93 to 34.76 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 7.26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: yield
- Remarks on result:
- other: 95% CL: 5.80 to 9.12 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 9.17 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 7.32 to 10.89 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.93 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: yield
- Remarks on result:
- other: 95% CL: 0.53 to 1.39 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 0.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: yield
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): control, 0.2, 0.6, 1.8 mg/L: normal size and shape of algal cells; 5.6 mg/L: a few algae cells of twice the size of algae cells compared to control; 50.0 mg/L: no changes, only very few cells
- Unusual cell shape: Irregular shaped and tear-shaped algae cells were observed at 16.7 mg/L.
- Colour differences: no changes observed
- Flocculation: no
- Adherence to test vessels: no
- Aggregation of algal cells: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: 72-h ErC50 2.09 mg/L (95% CL: 1.89 to 2.33 mg/L)
- Other: performed January 24 – 27, 2012 - Reported statistics and error estimates:
- Probit method calculations and analysis: Shapiro Wilk’s Test on Normal Distribution, Levene’s Test on Variance Homogeneity (with Residuals),
Williams Multiple Sequential t-test Procedure.
Any other information on results incl. tables
pH values
Test concentration [mg/L, nominal] |
pH |
|
0 h* |
72 h** |
|
Control |
7.22 |
9.09 |
0.2 |
7.49 |
8.93 |
0.6 |
7.47 |
8.84 |
1.8 |
7.47 |
8.27 |
5.6 |
7.75 |
8.02 |
16.7 |
8.79 |
7.85 |
50.0 |
9.56 |
7.84 |
*: pH measured in samples before division into replicates
**: pH measured in samples of pooled replicates
Growth Rate and Yield (mean values and standard deviations, SD)
Test concentration [mg/L, nominal] |
Growth Rate |
Yield |
||||
0-24 h |
24-48 h |
48-72 h |
0-72 h |
72 h |
||
0.0 (control) |
mean |
2.278 |
1.557 |
1.197 |
1.677 |
1.524 |
SD |
0.034 |
0.053 |
0.019 |
0.016 |
0.076 |
|
0.2 |
mean |
2.262 |
1.541 |
1.175 |
1.659 |
1.443 |
SD |
0.140 |
0.027 |
0.08 |
0.024 |
0.103 |
|
0.6 |
mean |
2.219 |
1.535 |
1.128 |
1.628 |
1.311 |
SD |
0.088 |
0.058 |
0.006 |
0.016 |
0.063 |
|
1.8 |
mean |
2.176 |
1.541 |
1.076 |
1.598 |
1.197 |
SD |
0.057 |
0.034 |
0.049 |
0.008 |
0.028 |
|
5.6 |
mean |
2.111 |
1.421 |
1.057 |
1.530 |
0.975 |
SD |
0.053 |
0.093 |
0.034 |
0.016 |
0.048 |
|
16.7 |
mean |
2.058 |
0.954 |
0.881 |
0.823 |
0.481 |
SD |
0.019 |
0.092 |
0.096 |
0.029 |
0.014 |
|
50.0 |
mean |
0.943 |
0.420 |
0.173 |
n.d. |
0.037 |
SD |
0.124 |
0.084 |
0.046 |
n.d. |
0.005 |
Inhibition of growth rate and yield of algae
Test concentration [mg/L, nominal] |
Inhibition [%] |
|
Growth rate |
Yield |
|
Control |
0.0 |
0.0 |
0.2 |
1.1 |
5.3 |
0.6 |
3.0 |
13.9 |
1.8 |
4.8 |
21.5 |
5.6 |
8.8 |
36.0 |
16.7 |
22.6 |
68.4 |
50.0 |
69.5 |
97.6 |
Concentration and stability of test item
Test concentration [mg/L, nominal] |
Measured concentration (n = 3) |
|||
0 h |
72 h |
|||
[mg/L] |
[% nominal] |
[mg/L] |
[% initial] |
|
0.0 (control) |
0.0 |
- |
0 |
- |
0.2 |
0.18 |
90.0 |
0.17 |
94.4 |
0.6 |
0.60 |
100.0 |
0.55 |
91.7 |
1.8 |
1.90 |
105.6 |
1.76 |
92.6 |
5.6 |
5.91 |
105.5 |
5.58 |
94.4 |
16.7 |
16.90 |
101.2 |
16.86 |
99.8 |
50.0 |
48.39 |
96.8 |
46.76 |
96.3 |
Effect values
Endpoint |
Growth rate |
Yield |
||||
Time of exposure |
Time of exposure |
|||||
24 h |
48 h |
72 h |
24 h |
48 h |
72 h |
|
EC50 |
42.43 (38.07 4 47.50) |
35.04 (32.39 – 38.09) |
31.66 (28.93 – 34.76) |
26.86 (21.10 – 34.46) |
11.58 (9.56 – 14.03) |
7.26 (5.80 – 9.12) |
EC20 |
22.75 (17.98 – 26.54) |
14.55 (12.69 – 16.28) |
14.03 (11.95 – 15.94) |
14.30 (8.72 – 18.62) |
3.95 (2.76 – 5.10) |
1.89 (1.24 – 2.56) |
EC10 |
16.42 (11.74 – 20.24) |
9.19 (7.56 – 10.74) |
9.17 (7.32 – 10.89) |
10.29 (5.18– 14.32) |
2.25 (1.38 – 3.15) |
0.93 (0.53 – 1.39) |
LOEC |
16.70 |
1.80 |
0.6 |
5.60 |
0.6 |
≤0.2 |
NOEC |
5.60 |
0.60 |
0.2 |
1.80 |
0.2 |
<0.2 |
Endpoint values of growth rate and yield based on nominal concentrations of test item [mg/L]
( - ) - 95% confidence interval
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.