Registration Dossier
Registration Dossier
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EC number: 224-832-0 | CAS number: 4511-42-6
Adsorption / desorption
Administrative data
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- GLP compliance:
- not specified
- Type of method:
- HPLC estimation method
- Media:
- other: HPLC column
Test material
- Reference substance name:
- L-(+)-lactic acid
- EC Number:
- 201-196-2
- EC Name:
- L-(+)-lactic acid
- Cas Number:
- 79-33-4
- IUPAC Name:
- 2-hydroxypropanoic acid
Constituent 1
- Radiolabelling:
- no
Study design
- Test temperature:
- Column temperature 35 degrees C
HPLC method
- Details on study design: HPLC method:
- EQUIPMENT
- Apparatus: Alliance Separation Module 2695
- Detection system: Dual lambda absorbance detector 2487
MOBILE PHASES
- Type: 30/70 methanol/water (neutral); 30/70 methanol/phosphate buffer (pH 2.0)
- Solutes for dissolving test and reference substances: water (lactic acid); methanol (phenol)
DETERMINATION OF DEAD TIME
- Method: by means of homologous series / by inert substances which are not retained by the column
REFERENCE SUBSTANCES
- Identity: Phenol
DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 10 µl; 209 ng (neutral), 135 ng (pH 2)
- Quantity of reference substances: 10 µl; 100 ng
REPETITIONS
- Number of determinations: all samples were measured in duplicate; controls were injected once
EVALUATION
- Calculation of capacity factors k': Not calculated
- Calculation of retention times: Not given other than in (single) chromatogram run printouts
- Determination of the log Koc value: <1.32 (i.e. < phenol log Koc)
Results and discussion
Adsorption coefficientopen allclose all
- Type:
- Koc
- Value:
- < 20.9
- Type:
- log Koc
- Value:
- < 1.32
Results: HPLC method
- Details on results (HPLC method):
- Lactic acid eluted before phenol; phenol has a designated log Koc of 1.32. No relevant differences in the results were observed at neutral pH and pH 2.0.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Lactic acid log Koc < 1.32.
- Executive summary:
The adsorption coefficient (Koc) of Lactic acid 93% aq. on soil and on sewage sludge was determined using high performance liquid chromatography (HPLC).
The Koc and log Koc value of the test substance at neutral pH and at pH 2 was <20.9 and <1.32, respectively.
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