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Toxicity to microorganisms

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Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Principles of method if other than guideline:
Method: other: growth inhibition test; Umweltbundesamt, Berlin: Bewertung wassergefaehrdender Stoffe. Erarbeitet von der ad-hoc-Arbeitsgruppe 1 "Bewertung wassergefaehrdender Stoffe"
GLP compliance:
yes
Remarks:
TNO Division of Technology for Society, Delft, The Netherlands
Analytical monitoring:
no
Test organisms (species):
Pseudomonas putida
Details on test conditions:
Type: aquatic
Duration:
18 h
Dose descriptor:
EC0
Effect conc.:
> 3 480 mg/L
Basis for effect:
growth inhibition
Remarks on result:
other: for neutralised concentrations (pH 7.6-7.8)
Duration:
18 h
Dose descriptor:
EC0
Effect conc.:
> 348 mg/L
Basis for effect:
growth inhibition
Remarks on result:
other: for unneutralised concentrations (pH>9)

RESULTS EXPOSED:
- Nominal/measured concentrations: Not reported
- EC0 (toxicity threshold): > 10000 mg/l for neutralised
concentrations (pH 7.6-7.8). Equivalent to >3480 mg active
matter/l. 
- EC0 (toxicity threshold): > 1000 mg/l for unneutralised
concentrations (pH > 9). Equivalent to > 348 mg active
matter/l.
RESULTS CONTROL: No effects
RESULTS TEST WITH REFERENCE SUBSTANCE: No reference
substance was tested

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, but no information on purity of test substance.
Principles of method if other than guideline:
Method: other: DIN 38412, Teil 27 (Bacterial oxygen consumption test)
GLP compliance:
yes
Remarks:
Henkel KGaA, Forschung Biologie Ökologie.
Test organisms (species):
Pseudomonas putida
Duration:
30 min
Dose descriptor:
EC0
Effect conc.:
3 454 mg/L

RESULTS: EXPOSED
- Nominal concentrations: 10000 mg product/l 
- Effect data (Immobilisation): EC0 10000 mg product/l (3454
mg active matter/l) (pH 11.1, at start 8.0), oxygen
consumption was reduced by 8.13% i.e.  10%
- Concentration / response curve: not reported
- Cumulative immobilisation: not reported
- Effect concentration vs. test substance solubility: not
reported
- Other effects: not reported
RESULTS CONTROL: not reported
RESULTS: TEST WITH REFERENCE SUBSTANCE
- Concentrations: not reported
- Results: not reported

Endpoint:
toxicity to microorganisms
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Justification for type of information:
Refer to category justification provided in IUCLID section 13.
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Duration:
18 h
Dose descriptor:
EC0
Effect conc.:
> 3 480 mg/L
Basis for effect:
growth inhibition
Remarks on result:
other: for neutralised concentrations (pH 7.6-7.8)
Remarks:
Source, key, 1344-09-8, Hanstveit 1989
Duration:
18 h
Dose descriptor:
EC0
Effect conc.:
> 348 mg/L
Basis for effect:
growth inhibition
Remarks on result:
other: for unneutralised concentrations (pH>9)
Remarks:
Source, key, 1344-09-8, Hanstveit 1989
Duration:
30 min
Dose descriptor:
EC0
Effect conc.:
3 454 mg/L
Remarks on result:
other:
Remarks:
Source, key, 1344-09-8, Kirch 1993

Description of key information

EC0 (18h, growth inhibition) > 10000 mg/L for neutralized concentrations (pH 7.6-7.8). Equivalent to > 3480 mg active matter/L (Pseudomonas putida)
EC0 (18h, growth inhibition) > 1000 mg/L for unneutralised concentrations (pH > 9). Equivalent to > 348 mg active matter/L (Pseudomonas putida)
EC0 (30min, oxygen consumption inhibition) 3454 mg/L

Key value for chemical safety assessment

Additional information

No study available on potassium silicate. Data are read-across to sodium silicate. The toxicity of a sodium silicate solution (MR 3.46, 34.8 wt%) has been determined with a growth inhibition test in compliance with German standards and GLP using the bacterium Pseudomonas putida (Hanstveit 1989). The 18 h toxicity threshold (EC10, 10 % inhibition) of a neutralised silicate solution of pH 7.6 - 7.8 was > 3480 mg active matter/L, the highest concentration tested, while for the unneutralised solution (pH 7.9 - 10.4) effects were found at concentrations above 348 mg active matter/L. In a further GLP guideline study complying with German standards corresponding to OECD 209, the toxicity to Pseudomonas putida was tested in an oxygen consumption inhibition test. Concentrations of a sodium silicate solution (MR 3.0, 34.54 wt%) of up to 3454 mg active matter/L at pH 8.0 - 11.1 did not cause toxic effects (Kirch 1993).