Registration Dossier
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EC number: 200-315-5 | CAS number: 57-13-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic plants other than algae
Administrative data
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 011
Materials and methods
- Principles of method if other than guideline:
- Automatic biotest ECOTOX.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Urea
- EC Number:
- 200-315-5
- EC Name:
- Urea
- Cas Number:
- 57-13-6
- Molecular formula:
- CH4N2O
- IUPAC Name:
- urea
- Test material form:
- not specified
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test organisms
- Test organisms (species):
- other: Euglena gracilis
- Details on test organisms:
- E. gracilis KLEBS, strain Z from University of Göttingen
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Total exposure duration:
- 24 h
- Remarks on exposure duration:
- 4 different time intervals: 0 h, 1 h, 6 h, 24 h
- Post exposure observation period:
- Recovery tests were performed with 3 concentrations for the 24 hour exposure group. The cells were transferred to deonized water and recovery was observed after 24 and 72 hours.
Test conditions
- Test temperature:
- 20 °C
- Nominal and measured concentrations:
- Test concentrations:
1,05; 1,7; 3; 2,5; 6; 10,5; 17; 25; 35; 45 g/l
Recovery group:
8,5; 17; 34 g/l - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 1 h
- Dose descriptor:
- EC50
- Effect conc.:
- 17.182 g/L
- Basis for effect:
- other: Inhibition of motility
- Remarks on result:
- other: 97% CL
- Duration:
- 6 h
- Dose descriptor:
- EC50
- Effect conc.:
- 14.945 g/L
- Basis for effect:
- other: Inhibition of motility
- Remarks on result:
- other: 99% CL
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 16.949 g/L
- Basis for effect:
- other: Inhibition of motility
- Remarks on result:
- other: 96% CL
- Duration:
- 1 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10.5 g/L
- Basis for effect:
- other: Inhibition of motility
- Remarks on result:
- other: 97% CL
- Duration:
- 6 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10.5 g/L
- Basis for effect:
- other: Inhibition of motility
- Remarks on result:
- other: 99% CL
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10.5 g/L
- Basis for effect:
- other: Inhibition of motility
- Remarks on result:
- other: 97% CL
Applicant's summary and conclusion
- Executive summary:
The effect of two commonly used fertilizers, DAP (diammonium phosphate) and urea was studied on the freshwater flagellate Euglena gracilis using the automatic biotest ECOTOX. NOEC and EC50 values for various parameters like motility, velocity, cell shape and gravitaxis were calculated. The NOEC and EC50 values obtained for DAP were much lower than those for urea; i.e. DAP showed a stronger inhibitory effect as compared to urea. The inhibition caused by DAP increased with increasing exposure time over 24 h but urea showed no augmentation with increasing exposure time. Application of DAP resulted in an increased pH and high concentrations of ammonia but urea did neither affect the pH nor affect the ammonia concentration. Recovery experiments in deionized water after urea application showed a reconstitution of motility after 72 h. After an application of 1.35 g L−1 (24 h EC50 for motility) DAP motility recovered after 72 h but motility did not recover when the concentration was doubled (2.7 g L−1). The EC50 values obtained were compared with the EC50/LC50 values reported for other aquatic organisms and were found to be comparable with the reported values.
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