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EC number: 231-959-5 | CAS number: 7782-50-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Deficiencies: max. duration of treatment only 0.5 hour, no batch number indicated.Although some aspects of the study do not meet up-to-date standards, the results are reliable and allow a scientific valid evaluation of this toxicological endpoint. New studies are therefore not required and should not be conducted due to animal welfare considerations.
Cross-reference
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Deviations:
- yes
- Remarks:
- Short exposure time (max. 30 min.)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Chlorine
- EC Number:
- 231-959-5
- EC Name:
- Chlorine
- Cas Number:
- 7782-50-5
- Molecular formula:
- Cl2
- IUPAC Name:
- dichlorine
- Details on test material:
- - Name of test material (as cited in study report): Chlorine - Analytical purity: 99.9 %- Impurities (identity and concentrations): Traces of Fe2(SO4)3- Lot/batch No.: Not stated - Stability under test conditions: Not stated- Traces of Fe2(SO4)3
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- Swiss
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS- Source: Charles River France SA, Saint Aubin les Elboeuf, France- Age at study initiation: Not stated- Weight at study initiation: mean weight 31.2 g (males); 25.3 g (females).
Administration / exposure
- Route of administration:
- inhalation: gas
- Type of inhalation exposure:
- whole body
- Vehicle:
- other: air
- Details on inhalation exposure:
- Duration of exposure:Group B, H, J, N, V and T: 10 min.Group R, E, K, C and Z: 30 min.
- Duration of exposure:
- >= 10 - <= 30 min
- Concentrations:
- Group B: 1680 mg/m3 (1.68 mg/L)Group C: 1757 ± 35 mg/m3 (1.757 mg/L)Group E: 1586 ± 47 mg/m3 (1.586 mg/L)Group H: 2186 ± 81 mg/m3 (2.186 mg/L)Group J: 2363 ± 81 mg/m3 (2.363 mg/L)Group K: 1665 ± 32 mg/m3 (1.665 mg/L)Group N: 3485 mg/m3 (3.485 mg/L)Group R: 1328 ± 55 mg/m3 (1.328 mg/L)Group T: 4798 mg/m3 (4798 mg/L)Group V: 3826 ± 38 mg/m3 (3.826 mg/L)Group Z: 1870 ± 21 mg/m3 (1.87 mg/L)
- No. of animals per sex per dose:
- 5 per sex and group
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days- Frequency of observations and weighing: performed on days 1, 2, 4, 7 and 14- Necropsy of survivors performed: yes- Other examinations performed: clinical signs, Mortalities, gross autopsy, body weights, weight of kidneys, liver, and lungs, lung and conductive airways were preserved for microscopic examination
- Statistics:
- Method of determination of LD50:Response-concentration-time relationship was used to estimate the LC50 values
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- 3 064 mg/m³ air
- 95% CL:
- 2 560 - 3 657
- Exp. duration:
- 10 min
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- 1 462 mg/m³ air
- 95% CL:
- 1 198 - 1 671
- Exp. duration:
- 30 min
- Mortality:
- For mortalities please refer to table below.
- Clinical signs:
- other: Wet nares, bubble formation and nasal discharge (mainly in the highest concentration group)
- Body weight:
- Both male and female mice lost body weight during the first 2 days after exposure which is a common finding in this type of experiment. After day 4 most animals gained body weight again but weight gain was generally low.
- Gross pathology:
- Not stated
- Other findings:
- Organ weights:Relative lung weights were generally increased. For kidneys and liver no dose related effects were seen.
Any other information on results incl. tables
Mortality rates
Group | Concentration | Exposure time (min.) | Mortality rate (%) | Day of death (number) |
B | 1.680±0 | 10 | 0 | - |
H | 2.186±81 | 10 | 0 | - |
J | 2.363±81 | 10 | 30 | 6(1); 13(2) |
N | 3.485±0 | 10 | 40 | 0(2); 10(1); 13(1) |
V | 3.826±38 | 10 | 100 | 0(8); 1(2) |
T | 4.798±0 | 10 | 100 | 0(10) |
R | 1.328±55 | 30 | 40 | 0(1); 8(2); 13(1) |
E | 1.586±47 | 30 | 70 | 0(4); 8(1); 9(1); 11(1) |
K | 1.665±32 | 30 | 60 | 0(2); 1(2); 9(1); 11(1) |
C | 1.757±35 | 30 | 90 | 0(5); 10(3); 13(1) |
Z | 1870±21 | 30 | 70 | 0(3); 1(2); 8(2) |
a SD: Standard deviation
Applicant's summary and conclusion
- Interpretation of results:
- Category 3 based on GHS criteria
- Remarks:
- Migrated information
- Conclusions:
- Most of the effects of chlorine occurred in the alveoli. Exposure to chlorine resulted additionally in changes in the conductive airways in some animals.The LC50 value for 30 min. exposure was derived at 1.462 mg/L.
- Executive summary:
MATERIALS AND METHODS
11 groups of 5 male and 5 female mice were exposed to different concentrations of chlorine for 10, or 30 min. After exposure the animals were observed for 14 days (signs of intoxication, mortalities, and body weights on days 1,2,4,7 and 14). After the observation period they were killed and subjected to a gross post mortem examination. The lung and conductive airways from selected animals were preserved for microscopic examination. Weight of kidneys, liver, and lungs was determined.
RESULTS AND DISCUSSION
The clinical observation of nasal discharge with bubble formation indicates an increase in discharge of mucus from the respiratory tract. This can be explained by the irritative nature of Cl2. Foamy discharges usually indicate lung oedema. Swollen lungs possibly indicative of oedema were observed in animals which were examined closely after spontaneous death and after the scheduled kill in the satellite groups. However, for appraisal of acute oedema the lungs have to be cut to show the superfluous fluid which was not done since it is incompatible with fixation of the lungs for microscopic examination. With reference to the LC50 derivation no sex difference was observed. For pathological examinations please refer to 4.2. The loss of body weight is a common finding in this type of experiment. After day 4 most animals gained body weight again but weight gain was low. Relative organ weights were generally increased. The increase was positively correlated with the concentration level and the duration of exposure. For kidneys and liver no dose related effects were seen.
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