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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP Guideline study with restictions
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Amines, tallow alkyl
EC Number:
263-125-1
EC Name:
Amines, tallow alkyl
Cas Number:
61790-33-8
IUPAC Name:
61790-33-8
Constituent 2
Reference substance name:
Amines, tallow alkyl
IUPAC Name:
Amines, tallow alkyl
Details on test material:
- Name of test material (as cited in study report): Tallowalkylamine

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Dispersion treatment: Stock solution was 10 mg/L. The test item was applied to natural river water. This solution was treated for 30 minutes with ultrasound at 40 °C.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain: Daphnia magna STRAUS (Clone 5)
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu); Breeder DR.U.NOACK-LABORATORIEN, D-31157 Sarstedt, Germany
- Age at study initiation: 2 - 24 h old
- Feeding during test: The daphnids were not fed during the study.

HOUSING:
- Conditions: In 2-3 L glass vessels with approximately 1.8 L culture medium, at 21 °C (temperatures of 20 - 25 °C are tolerated), in an
incubator, 16 h illumination, illumination strength max. 20 μE⋅m-2 ⋅s-1.
- Culture medium: Elendt M4, according to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaCO3/L.
- Type and amount of food: Feeding ad libitum with a mix of Desmodesmus subspicatus and Chlorella vulgaris, with an algae cell density of > 106 cells/mL.
- Feeding frequency: 5x weekly



Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d

Test conditions

Test temperature:
20±2 °C
Nominal and measured concentrations:
Nominal concetrations: 0.013, 0.032, 0.08, 0.2, 0.5 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, fill volume: Glass beakers (5 cm ID x 8 cm H), 100 mL; Volume of the study medium is 50 mL
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Renewal rate of test solution: 3 times per week ( monday, wednesday and friday)



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A natural river water of agricultural backround, middle reach of the river "Böhme", Lower-Saxony was used.
Test water parameter:
pH: 8.2
Conductivity [μS/cm]: 397
DOC [mg C/L]: 7.3
TOC [mg C/L]: 9.9
Ammonium-N [mg N/L]: 0.141
Nitrate-N [mg N/L]: 2.38
o-Phosphate-P [mg P/L]: 0.031
Total Phosphate [mg P/L]: 0.128
Suspended Matter [mg/L]: 17.4
Total Hardness [mg CO3/L]: 91.3
- Culture medium different from test medium: yes


OTHER TEST CONDITIONS
- Photoperiod: 16/8 hours light dark cycle
- Light intensity: Diffuse light, illumination range max. 20 μE⋅m-2 ⋅ s-1


EFFECT PARAMETERS MEASURED: reproduction and mortality


Reference substance (positive control):
yes
Remarks:
potassiumdichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.013 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
0.032 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
0.24 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Results with reference substance (positive control):
- Results with reference substance valid? yes
- Relevant effect levels: EC50 = 2.1 (24h)

Any other information on results incl. tables

Table 1: Summary of all test item related effects in the test concentrations

Effects

Nominal concentrations (mg/L)

0.013

0.032

0.08

0.02

0.5

Reproduction rate

--

++

++

++

++

Stillborn juveniles

--

--

++

++

--

Aborded eggs

--

--

--

--

--

immobilization

--

--

--

--

++

Dry weight

--

--

--

--

++

Lenght

--

--

--

--

++

Males/winter eggs

--

--

--

--

--

Summary:

--

++

++

++

++

-- no significant effect

++ significant effect

Applicant's summary and conclusion