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EC number: 231-639-5 | CAS number: 7664-93-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Published investigative non-guideline, non-GLP study.
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- 96-hour LC50 measurement
- GLP compliance:
- no
- Remarks:
- older, published study
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable - Analytical monitoring:
- yes
- Details on sampling:
- Not detailed
- Vehicle:
- no
- Details on test solutions:
- Not applicable
- Test organisms (species):
- Lepomis macrochirus
- Details on test organisms:
- 4 cm tail length obtained from a state fish hatchery in Louisiana, USA. Maintained in groups of 30 in 38 L aquaria (51 x 32 x 27 cm) at pH 6.5 and temperature of 21 to 25 degrees C. Lighting was continuous and fish were fed tropical fish food or brine shrimp each evening.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- Fish were exposed to each ph for 96 hours.
- Post exposure observation period:
- Not applicable
- Hardness:
- Not reported
- Test temperature:
- Presumed to be between 21 and 25 degrees C
- pH:
- pH was changed as appropriate during the study.
- Dissolved oxygen:
- Not reported
- Salinity:
- Not aplicable
- Nominal and measured concentrations:
- Test medium were amended with sulphuric acid to prepare the required pH of the test media.
- Details on test conditions:
- Not reported
- Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 16 - < 28 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- There was no mortality at pH 7.5, 5.0, 4.5, 4.0 or 3.5. Mortality was 100% at pH 3.25 and 3.0. The 96 hour LC50 was therefore between pH 3.25 and 3.5. Therefore the 96 h LC50 was between 16 and 28 mg/L.
No mortality was observed when the pH of the 3.0 aquarium was returned to pH 7.5 and fish were added to the aquaria.
Rate constants for locomotor activity before (pH7.5) and after (pH 4.0) exposure to sulphuric acid were 0.118 and 0.026 respectively, indicating that fish after acid exposure were only one quarter as active at pH 4.0 as they were at pH 7.5. - Results with reference substance (positive control):
- No applicable.
- Reported statistics and error estimates:
- Not applicable
- Sublethal observations / clinical signs:
The 96 hour LC50 for sulphuric acid to bluegill sunfish was determined to be between pH 3.25 and 3.5, equivalent to 16 to 28 mg/L.
- Validity criteria fulfilled:
- not applicable
- Remarks:
- not specified
- Conclusions:
- Based on the reported information an acceptable assessment of acute toxicity to fish was achieved.
- Executive summary:
An acute toxicity with bluegill sunfish (Lepomis macrochirus) was conducted in which fish were exposed to series of pH decreasing from 7.5 to 3.25. There was no mortality at pH values of 7.5, 5.0, 4.5, 4.0 or 3.5 and 100% mortality at 3.25.
Rate constants for locomotor activity before (pH7.5) and after (pH 4.0) exposure to sulphuric acid were 0.118 and 0.026 respectively, indicating that fish after acid exposure were only one quarter as active at pH 4.0 as they were at pH 7.5.
The 96 hour LC50 for sulphuric acid to bluegill sunfish was determined to be between pH 3.25 and 3.5, equivalent to 16 to 28 mg/L.
Reference
Description of key information
A published paper on the effects of sulphuric acid on a freshwater fish, bluegill sunfish (Lepomis macrochirus) has been summarised and a 96 hour LC50 obtained.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- > 16 - < 28 mg/L
Additional information
An acute toxicity study performed with bluegill sunfish (Lepomis macrochirus) was conducted, in which fish were exposed to series of pH values decreasing from 7.5 to 3.25. There was no mortality at pH values of 7.5, 5.0, 4.5, 4.0 or 3.5 and 100% mortality at 3.25. The 96 hour LC50 for sulphuric acid to bluegill sunfish was determined to be between pH 3.25 and 3.5, equivalent to 16 to 28 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

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