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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Published investigative non-guideline, non-GLP study.
Reason / purpose for cross-reference:
reference to same study
Qualifier:
no guideline available
Principles of method if other than guideline:
96-hour LC50 measurement
GLP compliance:
no
Remarks:
older, published study
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Analytical monitoring:
yes
Details on sampling:
Not detailed
Vehicle:
no
Details on test solutions:
Not applicable
Test organisms (species):
Lepomis macrochirus
Details on test organisms:
4 cm tail length obtained from a state fish hatchery in Louisiana, USA. Maintained in groups of 30 in 38 L aquaria (51 x 32 x 27 cm) at pH 6.5 and temperature of 21 to 25 degrees C. Lighting was continuous and fish were fed tropical fish food or brine shrimp each evening.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Remarks on exposure duration:
Fish were exposed to each ph for 96 hours.
Post exposure observation period:
Not applicable
Hardness:
Not reported
Test temperature:
Presumed to be between 21 and 25 degrees C
pH:
pH was changed as appropriate during the study.
Dissolved oxygen:
Not reported
Salinity:
Not aplicable
Nominal and measured concentrations:
Test medium were amended with sulphuric acid to prepare the required pH of the test media.
Details on test conditions:
Not reported
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 16 - < 28 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
There was no mortality at pH 7.5, 5.0, 4.5, 4.0 or 3.5. Mortality was 100% at pH 3.25 and 3.0. The 96 hour LC50 was therefore between pH 3.25 and 3.5. Therefore the 96 h LC50 was between 16 and 28 mg/L.

No mortality was observed when the pH of the 3.0 aquarium was returned to pH 7.5 and fish were added to the aquaria.

Rate constants for locomotor activity before (pH7.5) and after (pH 4.0) exposure to sulphuric acid were 0.118 and 0.026 respectively, indicating that fish after acid exposure were only one quarter as active at pH 4.0 as they were at pH 7.5.



Results with reference substance (positive control):
No applicable.
Reported statistics and error estimates:
Not applicable
Sublethal observations / clinical signs:

The 96 hour LC50 for sulphuric acid to bluegill sunfish was determined to be between pH 3.25 and 3.5, equivalent to 16 to 28 mg/L.

Validity criteria fulfilled:
not applicable
Remarks:
not specified
Conclusions:
Based on the reported information an acceptable assessment of acute toxicity to fish was achieved.
Executive summary:

An acute toxicity with bluegill sunfish (Lepomis macrochirus) was conducted in which fish were exposed to series of pH decreasing from 7.5 to 3.25. There was no mortality at pH values of 7.5, 5.0, 4.5, 4.0 or 3.5 and 100% mortality at 3.25.

Rate constants for locomotor activity before (pH7.5) and after (pH 4.0) exposure to sulphuric acid were 0.118 and 0.026 respectively, indicating that fish after acid exposure were only one quarter as active at pH 4.0 as they were at pH 7.5.

The 96 hour LC50 for sulphuric acid to bluegill sunfish was determined to be between pH 3.25 and 3.5, equivalent to 16 to 28 mg/L.

Description of key information

A published paper on the effects of sulphuric acid on a freshwater fish, bluegill sunfish (Lepomis macrochirus) has been summarised and a 96 hour LC50 obtained.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
LC50
Effect concentration:
> 16 - < 28 mg/L

Additional information

An acute toxicity study performed with bluegill sunfish (Lepomis macrochirus) was conducted, in which fish were exposed to series of pH values decreasing from 7.5 to 3.25. There was no mortality at pH values of 7.5, 5.0, 4.5, 4.0 or 3.5 and 100% mortality at 3.25. The 96 hour LC50 for sulphuric acid to bluegill sunfish was determined to be between pH 3.25 and 3.5, equivalent to 16 to 28 mg/L.

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