Registration Dossier
Registration Dossier
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EC number: 226-109-5 | CAS number: 5281-04-9
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Calcium 4-[(5-chloro-4-methyl-2-sulphonatophenyl)azo]-3-hydroxy-2-naphthoate
- EC Number:
- 230-303-5
- EC Name:
- Calcium 4-[(5-chloro-4-methyl-2-sulphonatophenyl)azo]-3-hydroxy-2-naphthoate
- Cas Number:
- 7023-61-2
- Molecular formula:
- C18H13ClN2O6S.Ca
- IUPAC Name:
- calcium 4-[(5-chloro-4-methyl-2-sulfonatophenyl)diazenyl]-3-hydroxy-2-naphthoate
- Test material form:
- solid: nanoform
Constituent 1
- Specific details on test material used for the study:
- - Storage condition of test material: at room temperature at abput 20 °C, away from direct sunlight
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil: the test substance was mixed directly with a portion of sand prior to addition to the artificial soil. For the preparation of the sand/test item mixtures, the amounts of 34.8, 69.5, 139, 278 and 556 mg of the test were weighed by an analytical balance and mixed into 10 g of sand each by means of a roller mixer. The sand/test item mixtures were prepared immediately before the start of the test. Each replicate was quantitatively incorporated into 556 g of artificial soil (dry weight) by intensely mixing.
Test organisms
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: earthworm
- Source: obtained from a breeding stock maintained at RCC
- Age at test initiation: adults with clitellum approx. 4 months old
- Weight at test initiation (mean range): 350 - 432 mg
HOUSING
- Feeding: horse manure, potatoes
ACCLIMATION
- Acclimation period: one day
- Acclimation conditions: artificial soil and test temperature
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
Test conditions
- Test temperature:
- 18 - 21 °C
- pH:
- - Initial pH = 5.8
- pH at test end: 5.9 - 6.2 - Moisture:
- - Initail moisture content: 35%
- Moisture at test end: 33-34% - Details on test conditions:
- TEST SYSTEM
- Test container: cylindrical glass vessels (diameter 10 cm, height 14 cm) covered with glass lids
- Amount of soil or substrate: 556 g artificial soil dry weight
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 5
- No. of replicates per control: 5
OTHER TEST CONDITIONS
- Photoperiod: coninuous illumination
- Light intensity: 526 - 631 lux
EFFECT PARAMETERS MEASURED:
- Mortality and symptoms of toxicity: after 7 and 14 days
- Body weight after: 0 and 14 days
- Soil moisture after: 0 and 14 days
- pH: after 0 and 14 days
- Temperature: continuously
TEST CONCENTRATIONS
- Range finding study: yes - Nominal and measured concentrations:
- Nominal test concentrations: control, 62.5, 125, 250, 500, 1000 mg/kg dry soil (based on range-finding study)
- Reference substance (positive control):
- yes
- Remarks:
- 2-chloroacetamide (test is performed once a year)
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 14 d
- Dose descriptor:
- LC0
- Effect conc.:
- >= 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 14 d
- Dose descriptor:
- LC100
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Mortality at end of exposure period: none
- Abnormal behaviour: none
- Symptoms of toxicity: none
- Body weight: not affected
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- No toxic effects have been observed in studies with soil living organisms.
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