Pentamethylenediamine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Read-across

GLP compliance:

no

Test type:

fixed concentration procedure

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-aminopropyldimethylamine

EC Number:

203-680-9

EC Name:

3-aminopropyldimethylamine

Cas Number:

109-55-7

Molecular formula:

C5H14N2

IUPAC Name:

N,N-Dimethyl-1,3-diaminopropane

Details on test material:

- Name of test material (as cited in study report): N,N-dimethylpropane-1,3-diamine
- Molecular formula (if other than submission substance): C5H14N2
- Molecular weight (if other than submission substance): 102.1781
- Smiles notation (if other than submission substance): CN(C)CCCN
- InChl (if other than submission substance): InChI=1/C5H14N2/c1-7(2)5-3-4-6/h3-6H2,1-2H3
- Structural formula attached as image file (if other than submission substance): see Fig.2
- Substance type: organic
- Physical state: liquid

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Dr. K. Thomae GmbH, Biberach, Germany
- Age at study initiation: 8-9 weeks
- Weight at study initiation: male: 255 ± 7 g, female: 183 ± 7 g
- Housing: groups of five per cage
- Diet: KLIBA rat/mouse laboratory diet ad libitum
- Water: ad libitum



ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 24 °C
- Humidity (%): 30 - 70 %
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

nose/head only

Vehicle:

other: unchanged (no vehicle)

Analytical verification of test atmosphere concentrations:

yes

Remarks:

gas chromatography

Duration of exposure:

4 h

Concentrations:

4.31 mg/L

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of weighing: before the beginning of the test, after 7 days and at the end of the observation period.
- Frequency of observations: several times during exposure and at least once on each workday in the observation period.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights

Statistics:

The statistical evaluation of the concentration/effect relationship was carried out on the basis of the binomial test in accordance with tables of the BASF Computer Center.

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 4.31 mg/L air

Exp. duration:

4 h

Remarks on result:

other: no mortality occured

Effect levels 2

Sex:

male/female

Dose descriptor:

LC0

Effect level:

4.31 mg/L air

Exp. duration:

4 h

Remarks on result:

other: no mortality occured

Mortality:

No mortality occured.

Clinical signs:

other: During exposure: animals showed immediately escape attempts, eyelid closure, accelerated respiration and after 15 min restlessness. After 2 h irregular respiration and closed eyelids were noted. After exposure and during observation period: up to 24 acce

Body weight:

The body weight gain of the male rats in the test group, compared with a historical. control collective, was not affected by the substance over the total observation period.
The body weight gain of the female rats in the test group, compared with a historical control collective, was slightly retarded in the second week of the observation period.

Gross pathology:

No pathologic findings noted.

Any other information on results incl. tables

Body weight:

Mean body weight (in g)	male			female
	day 0	day 7	day 14	day 0	day 7	day 14
Test group	255	280	319	183	195	207
Historical control group	248	286	318	177	196	211

The animals exhibited distinct eye irritation at the day of exposure which repressed during the recovery period (corneal stipplings, fundus not visible). There is indication that the test substance causes local irritation to exposed tissues, including respiratory tract.

Although the limit concentration of 5 mg/L was not fully met, the test is judged sufficient to characterize the acute inhalation toxicity of the substance as virtually nontoxic.