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EC number: 223-267-7 | CAS number: 3794-83-0
Effects ofEtidronicacidhydrate(Batch no.A3495) on mortality, biomass and the reproductive potential of the earthworm species Eisenia fetida (Annelida, Lumbricidae) were determined according to the Guidelines DIN ISO 11268-2 (1998), OECD 222 (2004) and BBA (1994). The study was conducted under static conditions over 8 weeks with the test item concentrations 62.5, 125, 250, 500 and 1000 mg test item/kg soil dry weight, which were mixed into artificial soil containing 10 % peat and a control using untreated artificial soil. 80 test organisms were inserted into 8 control replicates and 40 test organisms were divided into 4 replicates for each treatment (10 earthworms per replicate). They had an individual body weight between 0.32 and 0.54 g at experimental starting.
After 28 days of exposure in soil, no evident earthworm mortalities (< 10%) as well as no evident pathological symptoms or changes in the behaviour of adult earthworms were observed in all treatments. Moreover, no statistically significant differences in earthworm body weights were observed at all test item concentrations compared to the control. After further four weeks the reproduction rate (average number of juveniles produced) was 88 in the control and ranged between 46 and 115 in the test item treatment rates. Compared to the control, no statistically significant reduction of reproduction was observed at the test item concentrations 62.5, 125, 250 and 500 mg test item/kg soil dry weight. However, at 1000 mg test item/kg soil dry weight the reproduction was statistically significantly reduced. Overall, the NOEC of the test item concerning mortality, biomass and reproduction of earthworms was determined to be 500 mg test item/kg soil dry weight. The EC50- value for reproduction was determined to be > 1000 mg test item/kg soil dry weight.
An earthworm study following OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei)) was conducted with etidronic acid (CAS 2909-21-4). The overall NOEC for etidronic acid was determined as 500 mg/Kg. This corresponds to a NOEC of 714 mg/kg for tetrasodium (1-hydroxyethylidene)bisphosphonate (CAS 3794-83-0) after molecular weight correction. The results of the new study well support the previously available results of a reliability 2 study on earthworm toxicity which determined a 14 d LC50 value of >1000 mg/kg for the effects of HEDP sodium salt on the terrestrial worm E. foetida. It is also informative regarding the validity of the equilibrium partitioning approach for similar substances.
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