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EC number: 305-962-8 | CAS number: 95370-96-0
Table 1. Nitrate-N Formation Rate: NOEC-, LOEC and ECx-Values
mg/kg soil dry weight
95 % confidence interval
<62.5 - 145
154 - 213
<62.5 - 89.1
135 - 206
369 - 442
Table 2. Inhibition of Inorganic-N Formation Rates
Nitrate-N Formation Rate
Test concentration Inhibition [%] compared to control
[mg/kg soil dry weight]
Inhibition [%] compared to Control
The effects of Soybeanoil, epoxidized on the metabolic activity of the nitrogen-N formation rate (nitrate) soil micro-organisms was determined according to OECD Guideline 216 (2000) over a 28 day period. Nominal test concentrations were 62.5, 125, 250, 500 and 1000 mg/kg soil dry weight. The effects of Soybeanoil, epoxidized on the metabolic activity of the nitrogen-N formation rate (nitrate) was measured on the day of treatment (day 0) and after 7, 14 and 28 days. Untreated soil was tested as the control under the same test conditions as the test item replicates. After 7 days of exposure the EC25-value for inhibition of the nitrate-N formation rate in soil was under the lowest test item concentration of 62.5 mg/kg soil dry weight. The EC50-value was determined to be 76.6 mg/kg soil dry weight with a corresponding NOEC of <62.5 mg/kg dw. After 14 days of exposure the EC25- and EC50-values for inhibition of the nitrate-N formation rate were 109 and 169 mg/kg soil dry weight, respectively with a corresponding NOEC of 62.5 mg/kg dw. After 28 days of exposure the EC25-value for inhibition of the nitrate-N formation rate in soil was 182 mg/kg soil dry weight. The 28 day EC50-value was 402 mg/kg soil dry weight with corresponding LOEC and NOECs of 62.5 and <62.5 mg/kg dw soil respectively. Test validity criteria were fulfilled
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