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Ecotoxicological information

Toxicity to terrestrial plants

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Administrative data

Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2015-01-13 to 2015-05-06
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP Study according to OECD guidelines
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report Date:
2015

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
Deviations:
no
Principles of method if other than guideline:
N/A
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Details on test material:
Soybeanoil, epoxidized is a clear, viscous, colourless liquid with 100% purity.
Batch number 2014234102
Expiry date 2015-12-02
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
N/A

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
The toxic effects of the test item were determined on day 7, 14 and 21 (onion and carrot additionally on day 28) by visual observations (number of emerged seedlings and phytotoxic effects) and on day 21 (onion and carrot day 28) by shoot height and shoot fresh weight determination. The rating of the treated plants was done in relation to the control plants. Observations included all variations, either inhibitory or stimulatory, between the treated test replicates and the untreated control replicates. At the end of the study the shoot heights (in cm) and the shoot fresh weights of the shoots (in g) were measured additionally, directly after cutting the plants at the soil surface. The shoot height was measured per plant. Average mean values per replicate were calculated. The shoot fresh weight was determined for all plants from the same pot and considered as one replicate.

.

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:
The test item was incorporated into the soil in which the seeds were sown afterwards. The test container was bottom watered with nutrient solution as needed.
A 2:1 mixture of natural soil LUFA 2.2 and quartz sand was used.
Grain size: <2 mm
Carbon content: 0.83 and 1.0 %
pH-value: 5.45 ± 0.23 and 5.30 ± 0.04

Test organisms

open allclose all
Species:
Avena sativa
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
Tradename Ivory, Origin; Nordsaat Saatzucht GMBH, Saatzucht Langenstein, Böhnshauser Straße 1, 38895 Langenstein, Germany.
Species:
Allium cepa
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
Tradename Exhibition, Origin; Hild Samen GMBH, Postfach1161, 71666 Marbach, Germany.
Species:
Beta vulgaris
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Tradename Beretta Oko, Origin; KWS Saat AG / KWS Mais GMBH, Grimsehlstraße 31, 37574 Einbeck, Germany.
Species:
Brassica napus
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Tradename Kadore, Origin; KWS Saat AG / KWS Mais GMBH, Grimsehlstraße 31, 37574 Einbeck, Germany.
Species:
Daucus carota
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Tradename Sugarsnax 54, F1, Origin; Hild Samen GMBH, Postfach1161, 71666 Marbach, Germany.
Species:
Glycine max (G. soja)
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Tradename Merlin, Origin; Saatbau Linz, A-4021 Linz, Schimmerstraße 19, P.O.B. 317, Austria.

Study design

Test type:
seedling emergence toxicity test
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
21 d
Remarks:
Except for onion and carrot which was 28 days
Post exposure observation period:
No post exposure observation.

Test conditions

Test temperature:
Nominal range 12 - 32 °C, measured ranged between 17 - 22°C for A (oats, rape, soybean) and 15 - 21°C for B (onion, sugar beet and carrot)
pH:
N/A
Moisture:
Nominal range - 45 - 95 % relative humidity (RH), measured range 25 - 80% RH for A and 30-100% RH for B. The RH as occasionally <45% but is not considered to have influenced the plant growth.
Details on test conditions:
Non-porous plastic container (standard flower pots) with a diameter of ca. 12 cm were used. The pots were labelled with the study number and all necessary additional information to ensure unique identification. The seeds were stored dark at the test facility at room temperature (22 ± 10 °C) and protected from moisture until use. The test container was filled each with the same amount of treated soil and seeding holes were made with a seedling pistil. One seed was given into each hole and covered with soil. During cultivation the test container was bottom watered and fertilized as necessary.8 replicates were used per test concentration and control, tested in a randomised block design. 5 seeds were used per test container.
Nominal and measured concentrations:
Nominal test concentrations: 1000, 500, 250, 125, 62.5 mg/kg DW
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Species:
Avena sativa
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Allium cepa
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Beta vulgaris
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Brassica napus
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Daucus carota
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Glycine max (G. soja)
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Avena sativa
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Allium cepa
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Beta vulgaris
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Brassica napus
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
909 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: 786->1000
Species:
Daucus carota
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Glycine max (G. soja)
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Details on results:
At the end of the exposure period EC50 values for all plant species based on seedling emergence were greater than 1000 mg/kg dry weight (dw) soil, corresponding NOEC's were 500 mg/kg dw soil for onion, sugar beet and rape and 1000 mg/kg dw for oats, carrot and soybean. Based on growth EC50 values were >1000 mg/kg dw soil for all species apart from oilseed rape where the EC50 was calculated to be 909 mg/kg dw soil, corresponding NOECs were 250 mg/kg dw for rape, 500 mg/kg dw for oats, onion, sugar beet and carrot and 1000 mg/kg dw for soybean.

At test end slight test item related phytotoxic effects occurred for the plant species sugar beet at the test concentration 1000 mg/kg DW. For the plant species oats, onion, rape, carrot and soybean no test item related phytotoxic effects were determined up to the highest tested concentration of 1000 mg/kg DW
Results with reference substance (positive control):
N/A
Reported statistics and error estimates:
One Way Analysis of Variance (ANOVA) was carried out for the determination of statistically significant differences compared to control replicates. When running a One Way Analysis of Variance, a Normality test and an Equal Variance test were done first. P-values for both, Normality and Equal Variance test, are 0.05.

EC25- and EC50-values were calculated by sigmoidal dose- response regression. Calculation of the confidence intervals for EC-values was carried out using standard procedures.

Any other information on results incl. tables

Table1:          Shoot Height: NOEC, EC25- and EC50-Values with Confidence Range (p)

Species

NOEC
[mg/kg DW]

EC25
[mg/kg DW]

p = 95%
[mg/kg DW]

EC50
[mg/kg DW]

p = 95%
[mg/kg DW]

Oats

1000

> 1000

> 1000

Onion

500

> 1000

> 1000

Sugar beet

500

> 1000

> 1000

Rape

500

> 1000

> 1000

Carrot

1000

> 1000

> 1000

Soybean

1000

> 1000

> 1000

 

Table2:          Shoot Fresh Weight:NOEC, EC25- and EC50-Values with Confidence Range (p)

Species

NOEC
[mg/kg DW]

EC25
[mg/kg DW]

p = 95%
[mg/kg DW]

EC50
[mg/kg DW]

p = 95%
[mg/kg DW]

Oats

500

> 1000

> 1000

Onion

500

797

621 - >1000

> 1000

Sugar beet

500

723

542 - 879

> 1000

Rape

250

525

413 - 635

909

786 - >1000

Carrot

500

568

360 - 934

> 1000

Soybean

1000

> 1000

> 1000

Table3:          Number of Emerged Seedlings: NOEC, EC25- and EC50-Values with Confidence Range (p)

Species

NOEC
[mg/kg DW]

EC25
[mg/kg DW]

p = 95%
[mg/kg DW]

EC50
[mg/kg DW]

p = 95%
[mg/kg DW]

Oats

1000

> 1000

> 1000

Onion

1000

> 1000

> 1000

Sugar beet

1000

> 1000

> 1000

Rape

1000

> 1000

> 1000

Carrot

1000

> 1000

> 1000

Soybean

1000

> 1000

> 1000

-) Not determinable

 

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The toxic effects of Soybeanoil, epoxidized to 6 terrestrial plants was determined in seedling emergence and seedling growth tests over a period of 21 days (Onion and carrot: 28 days), from 2015-01-13 to 2015-05-06 according to OECD 208 (July 2006). The seedling emergence EC50 for all 6 plant species was >1000 mg/kg DW. The shoot height EC50 for all 6 plant species was >1000 mg/kg DW. The shoot weight EC50 value for all 6 plant species except rape was >1000 mg/kg DW. The EC50 for rape was 909 mg/kg DW. The results of the control for all plant species met the required validity criteria.


Executive summary:

The toxic effects of Soybeanoil, epoxidized to Avena sativa (oats), Allium sepa (onion), Beta vulgaris (sugar beet), Brassica napus (rape), Daucus carota (carrot) and Glycine max (soybean) was determined in seedling emergence and seedling growth tests over a period of 21 days (for onion and carrot exposure was over 28 days), according to OECD 208 (July 2006). Test systems comprised of two monocotyledons, oats (Poaceae) and onion (Liliaceae), and four dicotyledons, sugar beet (Amaranthaceae), rape (Brassicaceae), carrot (Umbelliferae) and soybean (Leguminosae). The test was conducted with the nominal test concentrations of 62.5, 125, 250, 500 and 1000 mg/kg dw (soil dry weight). The NOEC values, as well as the calculated EC-values were calculated for seedling emergence, shoot weight and shoot height. The seedling emergence EC50 for all plant species was >1000mg/kg dw, and the corresponding NOECs were all 1000 mg/kg dw. The shoot height EC50 for all six plant species was >1000mg/kg dw and the corresponding NOECs were 500 mg/kg dw soil for onion, sugar beet and rape and 1000 mg/kg dw soil for oats, carrot and soybean.   The shoot weight EC50 value for all six plant species except rape was>1000mg/kg dw, except for rape which was 909 mg/kg dw. The corresponding NOECs were 250 mg/kg dw for rape, 500 mg/kg dw for oats, onion, sugar beet and carrot and 1000 mg/kg dw for soybean. At test end slight test item related effects occurred for the plant species sugar beet at the test concentration 1000 mg/kg dw. For the plant species oats, onion, rape, carrot and soybean no test item related phytotoxic effects were determined up to the highest tested concentration of 1000 mg/kg dw. The results of the control for all plant species met the required validity criteria.