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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 16 - 20 September 1991
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP Guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
Principles of method if other than guideline:
As 2-amylanthraquinone is highly unstable in water under influence of light, the study was conducted under reduced light conditions (<100 lux).
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
- Nominal concentration: 200 µg/l.
- Sampling method: Samples of the test solutions were taken before and after renewal.
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Each day a stock solution was freshly prepared by dissolving about 20 mg 2-amylanthraquinone in 10 ml DMSO resulting in a
concentration of 2000 mg/l. A volume of 400 µl of this stock solution was added to 4 litre ISO water in duplicate.
- Controls: A control with DMSO in test medium and a control with test medium were included.
- Chemical name of vehicle: DMSO.
- Concentration of vehicle in test medium: 0.1 ml/l.
- Evidence of undissolved material: No.
Test organisms (species):
Poecilia reticulata
Details on test organisms:
TEST ORGANISM
- Source: commercial supplier, Rasbora, Veenendaal, The Netherlands.
- Weight at study initiation (mean and range, SD): 0.28 g/fish.
- Feeding during test: no.

ACCLIMATION
- Acclimation period: the fish arrived in the test laboratory on 24 July 1991.
- Acclimation conditions: same as test conditions.
- Type and amount of food: troutfeed.
- Feeding frequency: once daily.
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
no
Hardness:
250 mg/l as calcium carbonate.
Test temperature:
22 ± 1°C.
pH:
Controls: 7.8 - 8.1.
DMSO-controls: 7.8 - 8.1.
Test concentration (200 µg/l): 7.9 - 8.1.
Dissolved oxygen:
Controls: 8.4 - 8.8 mg/l
DMSO-controls: 8.4 - 8.8 mg/l
Test concentration (200 µg/l): 8.3 - 9.0 mg/l
Nominal and measured concentrations:
The mean measured concentration in the test solutions was 170 µg/l (see Table 1).
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type: open.
- Material: glass aquarium with a volume of 2 liter.
- Aeration: No.
- Renewal rate of test solution: the test solutions were renewed after 24, 48 and 72 hours.
- No. of organisms per vessel: 5 fish per test vessel.
- No. of vessels per concentration: 2 replicates.
- No. of vessels per control: 2 replicates.
- No. of vessels per vehicle control: 2 replicates.
- Biomass loading rate: 0.6 g fish/l.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted ISO water prepared by using distilled water.

OTHER TEST CONDITIONS
- Adjustment of pH: No.
- Photoperiod: 10h light/14 h dark.
- Light intensity: <100 lux.

EFFECT PARAMETERS MEASURED
Each 24 hours the following parameters were recorded: mortality, hyperactivity, hypoactivity, hyperventilation, uncontrolled movement, loss of
equilibrium and discolouring.

TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: no mortality occurred in preliminary tests at solubility level.
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 170 µg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
170 µg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
behaviour
Details on results:
See Table 2.
Sublethal observations / clinical signs:

Table 2: Mortality of fish during the study with 2-amylanthraquinone

Nominal test concentration (µg/l)

Number of dead fish per test vessel

Mortality at 96 hours

24 h

48 h

72 h

96 h

0 (control)

0

0

0

0

0 %

0 (control)

0

0

0

0

0 (DMSO-control)

0

0

0

0

0 %

0 (DMSO-control)

0

0

0

0

200

0

0

1

0

10 %

200

0

0

0

0

Validity criteria fulfilled:
yes
Conclusions:
Short term toxicity of 2-amylanthraquinone to fish was studied in laboratory conditions using Poecilia reticulata. The mean measured concentration in the test solutions was 170 µg/l. Since no adverse effects on fish were observed after 96h in the limit test, the LC50 was above 170 µg/L.

Description of key information

In a study performed according to OECD 203 test guidelines, Poecilia reticulata were exposed in a semi-static study for 96 hours to 2 -amylanthraquinone. The substance is unstable in water under influence of light, therefore the study was performed under reduced light.

The mean measured concentration in the test solutions was 170 μg/L (comparable to the water solubility). Since no adverse effects on fish were observed after 96h, the LC50 was determined to be >170 μg/L.

Key value for chemical safety assessment

Additional information