Reaction mass of 2-(1,1-dimethylpropyl)anthraquinone and 2-(1,2-dimethylpropyl)anthraquinone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 16 - 20 September 1991

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Principles of method if other than guideline:

As 2-amylanthraquinone is highly unstable in water under influence of light, the study was conducted under reduced light conditions (<100 lux).

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Nominal concentration: 200 µg/l.
- Sampling method: Samples of the test solutions were taken before and after renewal.

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Each day a stock solution was freshly prepared by dissolving about 20 mg 2-amylanthraquinone in 10 ml DMSO resulting in a
concentration of 2000 mg/l. A volume of 400 µl of this stock solution was added to 4 litre ISO water in duplicate.
- Controls: A control with DMSO in test medium and a control with test medium were included.
- Chemical name of vehicle: DMSO.
- Concentration of vehicle in test medium: 0.1 ml/l.
- Evidence of undissolved material: No.

Test organisms (species):

Poecilia reticulata

Details on test organisms:

TEST ORGANISM
- Source: commercial supplier, Rasbora, Veenendaal, The Netherlands.
- Weight at study initiation (mean and range, SD): 0.28 g/fish.
- Feeding during test: no.

ACCLIMATION
- Acclimation period: the fish arrived in the test laboratory on 24 July 1991.
- Acclimation conditions: same as test conditions.
- Type and amount of food: troutfeed.
- Feeding frequency: once daily.

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

no

Hardness:

250 mg/l as calcium carbonate.

Test temperature:

22 ± 1°C.

pH:

Controls: 7.8 - 8.1.
DMSO-controls: 7.8 - 8.1.
Test concentration (200 µg/l): 7.9 - 8.1.

Dissolved oxygen:

Controls: 8.4 - 8.8 mg/l
DMSO-controls: 8.4 - 8.8 mg/l
Test concentration (200 µg/l): 8.3 - 9.0 mg/l

Nominal and measured concentrations:

The mean measured concentration in the test solutions was 170 µg/l (see Table 1).

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: open.
- Material: glass aquarium with a volume of 2 liter.
- Aeration: No.
- Renewal rate of test solution: the test solutions were renewed after 24, 48 and 72 hours.
- No. of organisms per vessel: 5 fish per test vessel.
- No. of vessels per concentration: 2 replicates.
- No. of vessels per control: 2 replicates.
- No. of vessels per vehicle control: 2 replicates.
- Biomass loading rate: 0.6 g fish/l.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted ISO water prepared by using distilled water.

OTHER TEST CONDITIONS
- Adjustment of pH: No.
- Photoperiod: 10h light/14 h dark.
- Light intensity: <100 lux.

EFFECT PARAMETERS MEASURED
Each 24 hours the following parameters were recorded: mortality, hyperactivity, hypoactivity, hyperventilation, uncontrolled movement, loss of
equilibrium and discolouring.

TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: no mortality occurred in preliminary tests at solubility level.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 170 µg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

170 µg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

behaviour

Details on results:

See Table 2.

Sublethal observations / clinical signs:

Table 2: Mortality of fish during the study with 2-amylanthraquinone

Nominal test concentration (µg/l)	Number of dead fish per test vessel				Mortality at 96 hours
	24 h	48 h	72 h	96 h
0 (control)	0	0	0	0	0 %
0 (control)	0	0	0	0
0 (DMSO-control)	0	0	0	0	0 %
0 (DMSO-control)	0	0	0	0
200	0	0	1	0	10 %
200	0	0	0	0

Validity criteria fulfilled:

yes

Conclusions:

Short term toxicity of 2-amylanthraquinone to fish was studied in laboratory conditions using Poecilia reticulata. The mean measured concentration in the test solutions was 170 µg/l. Since no adverse effects on fish were observed after 96h in the limit test, the LC50 was above 170 µg/L.

Description of key information

In a study performed according to OECD 203 test guidelines, Poecilia reticulata were exposed in a semi-static study for 96 hours to 2 -amylanthraquinone. The substance is unstable in water under influence of light, therefore the study was performed under reduced light.

The mean measured concentration in the test solutions was 170 μg/L (comparable to the water solubility). Since no adverse effects on fish were observed after 96h, the LC50 was determined to be >170 μg/L.