Trisodium [3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-2-hydroxy-5-nitrobenzenesulphonato(3-)][2-hydroxy-3-[(2-hydroxy-1-naphthyl)azo]-5-nitrobenzenesulphonato(3-)]chromate(3-)

Toxicity to aquatic plants other than algae

Description of key information

ErC50 (7d): 22.7 mg/l

EyC50 (7d): 227 mg/l

Key value for chemical safety assessment

EC50 for freshwater plants:

22.7 mg/L

Additional information

In order to assess the potential toxicity to aquatic plants of the Acid Brown 365 (ABr365) the available data on the structural analogue 02, the variable sodium salt form were considered.

 

The inhibitory effects based on the average specific growth rate and the yield (absolute increase in plant size) to the duckweed Lemna minor was investigated over a period of 7 days. The test was performed at concentrations of 20, 200 and 2000 mg/l of the active ingredient. Taking into account a purity of 88.02 %, nominal concentrations of 22.7, 227 and 2270 mg/l, respectively, were prepared. No chemical analysis of the test concentrations were performed, nevertheless considering that the substance is a strong acid it is expected that it would be stable in water for the whole test period. The median effect concentrations of the test item to Lemna minor with respect to growth rate (ErC50) and yield (EyC50) were estimated to be 22.7–227 mg/l nominal concentration and the no-observed-effect concentrations were lower than 22.7 mg/l nominal concentration (REACH&Colours Kft, 2014).