Registration Dossier

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information

No exotoxicological information about Acid Brown 365 (ABr365) are available, thus a read across approach has been considered in order to assess the aquatic toxicity potential of the substance. Adequate chronic toxicity studies are not available, therefore the aquatic toxicity assessment and classification are based mainly on the short-term toxicity information on the structural analogous 01 and 02, both the variable sodium salt.

ABr365 has a log Kow lower than 4 (indicating a low potential for bioaccumulation) and it is expected to be not rapidly degradable.

According to the CLP Regulation (EC 1272/2008), acute aquatic toxicity is normally determined using a fish 96-hour LC50 , a crustacean species 48-hour EC50 and/or an algal species 72- or 96-hour EC50; nevertheless it is also specified in the Regulation that data on other species (e.g. Lemna spp.) shall also be considered if the test methodology is suitable. These species cover a range of trophic levels and taxa and are considered as surrogate for all aquatic organisms.

Data are available for fish (Brachydanio rerio and Leuciscus idus), Daphnia magna and Lemna minor.

According to the CLP Regulation (EC 12720/2008) criteria for the identification of the classification categories for hazardous to the aquatic environment, both the studies available on short-term toxicity to fish fixed the short-term effect level on fish between 10 and 100 mg/l; this classification category is confirmed by the Lemna study, in which the EC50 related to the effects on the grown rate is recorded in the same range of values.

In conclusion, the ABr365 is classified as Aquatic Chronic 3 (H412) according to the CLP Regulation (EC 1272/2008).